Sources for 5-HT2 receptors?

More Moustafa Gabr's questions See All

Which nucleases can degrade G-quadruplex ?

I am interested in the effect of different nucleases on G-quadruplex DNA. I see examples in the literature where exonucleases or endonucleases release G-rich sequence which consequently form...

10 November 2018 936 2 View

Melting temperature (Tm) of G-quadruplex DNA?

I am using G-rich oligonucleotides to form corresponding G-quadruplex DNA using a standard protocol. Specifically, I am using 22mer HTelo and 22mer human oncogene promoter with Tm of 57.7 and 61.8...

09 October 2018 9,505 9 View

Software for molecular docking with oligonucleotides?

I am trying to do molecular docking studies for metal complexes using crystal structure of oligonucleotide from PDB 2O1I. However, trying to perform it with Swissdock or Molegro failed because...

11 December 2017 1,099 3 View

Supercoiled plasmid DNA vs relaxed DNA differential binding ?

Few fluorescent dyes are reported to differentiate between supercoiled plasmid DNA and relaxed DNA by differential binding of the dye upon DNA relaxation ( ex: Picogreen). I was wondering how to...

11 December 2017 3,502 3 View

ITC in presence of DMSO ?

I am considering to perform ITC experiment for small molecule - peptide interaction. Both small molecule and peptide are insoluble in PBS buffer. I dissolve them in DMSO first then dilute with PBS...

10 November 2017 390 2 View

Quantification of ionic and covalent character ?

Are there any simple computational methods to quantify ionic and covalent characters of bond that are considered intermediate between ionic and covalent?. I thought of estimation of bond length...

09 October 2017 9,637 3 View

Solubility of Cobalt (II) compounds ?

I am wondering if there is any explanation for the dependence of solubility of Co(II) inorganic compounds in water on the counter ion. In particular, cobalt (II) cyanide is completely insoluble in...

08 September 2017 4,153 9 View

DLS measurements analysis ??

I am using Malvern Zetasizer Nano ZS instrument to perform DLS measurements. I am getting this statement at the end of all my measurements (means with ± 1 SD) like in the picture attached. Does...

08 September 2017 10,080 4 View

Experimental proof for intermolecular charge transfer in metal complexes

For some reported metal complexes as anion sensors in the literature, the proposed mechanism is that upon binding of the anion to the metal center, the extent of ICT from ligand to metal is...

07 August 2017 1,154 7 View

Fluorescent dye to differentiate between supercoiled and relaxed DNA ?

Are there any well established fluorescent dyes (alternatively to PicoGreen) that can differentiate between supercoiled and relaxed DNA, so that they are able to measure the difference in...

06 July 2017 1,630 3 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

How to understand this crystallographic phenomenon of low temperature crystals in zeolite?

During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...

06 August 2024 726 3 View

What is the reason I get 400nm nanoparticles using an extruder with 200 nm membrane for cell membrane coating?

I am trying to coat my micelles and PLGA based nanoparticles with cell membrane using an Avanti mini extruder according to literature . I use 200nm membrane but my particles end up to be 400nm And...

30 July 2024 3,294 3 View

Do some Staphylococcus aureus strains have in vitro antimycobacterial activity ?

Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?

29 July 2024 10,023 2 View

Does it exist some special technique for cutting ceramic membranes for Cross-sectional FESEM images?

Does it exist some special technique for cutting ceramic membranes for Cross-sectional FESEM or SEM images?

25 July 2024 5,482 2 View

How do we pick data for determination of Validation Acceptance Criteria?

Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....

23 July 2024 7,292 3 View

Looking for articles that provide guidance on doing Thematic Analysis (Braun & Clarke) as a team?

Does anyone know of any published articles that provide guidance on using TA with multiple team members? I am aware of one (see below), however, there are some disagreements in terms of some of...

23 July 2024 7,819 5 View

When I am trying to distill triethyl amine while drying it over calcium hydride. How do I design the setup to let hydrogen escape without losing NEt3?

I am new to research. The boiling point of triethyl amine (NEt3) is 89 degrees centigrade. I am skeptical that when I will let the hydrogen escape which is getting generated in situ, I will also...

21 July 2024 7,284 4 View

Karoly Liliom

Hi Moustafa,

I suggest you to get a proper plasmid for 5-HT receptor expression, so for example:

http://www.cdna.org/Serotonin-5-HT-c56.html

You can easily transfect HEK-293 or CHO (or many other available mammalian cell line) cells with this plasmid and make membrane preparation after two days of transfection. It is easy and reproducible.

Regards,

Karoly

Flavie Sicard

Check

http://www.bioxtal.com/

They should provide membrane expressing serotonin receptors