Which nucleases can degrade G-quadruplex ?

I am interested in the effect of different nucleases on G-quadruplex DNA. I see examples in the literature where exonucleases or endonucleases release G-rich sequence which consequently form...

10 November 2018 781 2 View

Melting temperature (Tm) of G-quadruplex DNA?

I am using G-rich oligonucleotides to form corresponding G-quadruplex DNA using a standard protocol. Specifically, I am using 22mer HTelo and 22mer human oncogene promoter with Tm of 57.7 and 61.8...

09 October 2018 9,328 9 View

Software for molecular docking with oligonucleotides?

I am trying to do molecular docking studies for metal complexes using crystal structure of oligonucleotide from PDB 2O1I. However, trying to perform it with Swissdock or Molegro failed because...

11 December 2017 924 3 View

Supercoiled plasmid DNA vs relaxed DNA differential binding ?

Few fluorescent dyes are reported to differentiate between supercoiled plasmid DNA and relaxed DNA by differential binding of the dye upon DNA relaxation ( ex: Picogreen). I was wondering how to...

11 December 2017 3,251 3 View

ITC in presence of DMSO ?

I am considering to perform ITC experiment for small molecule - peptide interaction. Both small molecule and peptide are insoluble in PBS buffer. I dissolve them in DMSO first then dilute with PBS...

10 November 2017 197 2 View

Quantification of ionic and covalent character ?

Are there any simple computational methods to quantify ionic and covalent characters of bond that are considered intermediate between ionic and covalent?. I thought of estimation of bond length...

09 October 2017 9,475 3 View

Solubility of Cobalt (II) compounds ?

I am wondering if there is any explanation for the dependence of solubility of Co(II) inorganic compounds in water on the counter ion. In particular, cobalt (II) cyanide is completely insoluble in...

08 September 2017 3,959 9 View

DLS measurements analysis ??

08 September 2017 9,897 4 View

Experimental proof for intermolecular charge transfer in metal complexes

For some reported metal complexes as anion sensors in the literature, the proposed mechanism is that upon binding of the anion to the metal center, the extent of ICT from ligand to metal is...

07 August 2017 1,000 7 View

Fluorescent dye to differentiate between supercoiled and relaxed DNA ?

Are there any well established fluorescent dyes (alternatively to PicoGreen) that can differentiate between supercoiled and relaxed DNA, so that they are able to measure the difference in...

06 July 2017 1,410 3 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

How to define tie constraint between rigid body and another part in dynamic explicit model of Abaqus?

Hi dear researchers I connected a rigid body into the part of my structure by tie constraint and then I added displacement to rigid body, in static general step every thing is fine and these two...

28 February 2021 3,442 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View