Jawad, i'm thinking about collecting a number of strains of bracket fungi (presumably the same species) from a determinated area, under a common substrate (all of them on the same species of tree). Vegetative incompatibility might be the right method to assess if every combination of two strains are the same species, but i'm not sure if this is useful to elucidate if they are the same individual. Knowing that, i should able to measure the area of the mycelia of a single fungi.
VCG is a classification at the subspecies level. You are not going to be sure if two isolates, belonging to the same VCG, are the same, albeit they are more closely related than two isolates of two different VCGs. However, it is a well-known (and relatively easy) tool that is going to give you useful ecologic information about your population; for example, in general, and depending on your species, you population could sexually reproduce if you have isolates of different MAT within a given VCG.
Específicamente, la idea era determinar si la misma especie y/o individuo (el eterno problema del individuo fúngico) de Phellinus sp. colonizó una extensión aproximada de 100 m2 donde habitan una decena de árboles de boldo (Peumus boldus). Una vez sabido esto, intentar extrapolar la información obtenida y estimar el tamaño del micelio de Phellinus sp.