Where can I find the following reagents?

Are there any vendors for the following chemicals in INDIA other than SIGMA Dibenzyl N,N-diisopropylphosphoramidite Tetrol-7 thanks in advance

09 October 2018 8,136 0 View

Synthesis of phosphorylated hydroxy acids?

I want to synthesis phospho lactate. Please suggest a protocol which is specific for lactic acid or in general for any hydroxy acid? this involves phosphorylation of the OH group. thanks in advance

09 October 2018 2,619 0 View

Reversible temperature dependent protein precipitation ?

I am working on a mesophilic protein (37 kDa and dimer in solution). I wanted to crystalize this protein and hence concentrated it to ~10 mg/mL. when kept on ice, the protein precipitated. kept...

07 August 2018 9,752 6 View

Leucine 60 to proline mutation in GFPmut3?

We wanted to tag a protein of interest with GFP mut3 version. upon generating the construct and sequencing it we found it has an additional mutation. A conserved leucine at 60th position had got...

07 August 2018 7,631 0 View

Soft and gel like protien crystals?

I am trying to crystallize a mesophilic enzyme and had set up screening for conditions by microbatch method. I saw huge structures in many conditions (images attached) after 3 weeks. But when we...

07 August 2018 7,397 0 View

Can in vivo studies be performed on Auxin induced degron P. berghei parasites in mice?

I am planning to generate a Auxin induced degron parasite (P. berghei) for a gene of interest. literature is available for only ex vivo studies in the presence and absence of auxin. Is it possible...

31 December 2017 7,719 0 View

"yeast secretory expression of heterologus proteins"?

08 September 2017 6,975 7 View

Generation of sequence alignment and Phylogenetic tree (for protein)

I did a blast search with a sequence of my protein of interest. I got around 4000 sequences in the result. How do I eliminate redundant sequences and multiple entries for the same organism from a...

05 June 2017 4,205 4 View

What is the probability of getting revertants after yeast transformation?

Hi, I am working with a yeast strain which is knockout for a gene of interest (AMP Deaminase) (gene replaced by g418 casette). this strain cannot grow on S-adenosyl methionine (SAM). I transformed...

01 February 2016 4,567 2 View

Query regarding serial dilution and spotting assay????

31 December 2015 9,880 3 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View

Alternative to mediation analysis?

I am currently working on my dissertation and want to explore the role of competence as a mediator between the effect of colour of clothes on voting intention (focus on Western countries)....

21 February 2021 8,609 1 View

How to calculate the fraction metabolized of a drug by an enzyme using clearance(ml/min) values of the metabolite formed?

I am trying to find the fraction metabolized of a drug by CYP and UGT enzymes. I am unable to get the values of fraction metabolized (Fm) directly from the literature. Can we calculate the Fm of a...

21 February 2021 5,379 2 View

Color of spiro-ometad film?

What does the color of a spiro-ometad film indicate? I got purple, green, red, even black color of the spiro film. Is there anything we can get from the preliminary color analysis(e.g. oxidation...

21 February 2021 1,831 4 View

How to visualise pathways from a phosphoproteomics dataset?

I am looking for something similar than Ingenuity Pathway Analysis (IPA) in which I can enter the proteins differentially phosphorylated and I can visualise the pathways these proteins are...

21 February 2021 8,923 4 View

Any information about dinitrosalisylic acid reagent end point color stability ?

I work on estimation of alpha amylase activity using dinitrosalisylic acid reagent If i put the reagent to stope the enzymatic reaction and allow the reagent to react with the produced reducing...

17 February 2021 7,402 4 View

Negative result on natural product sample cholinergic assay?

i have been doing cholinergic assay on natural product and for some of the samples, i get negative value for the inhibition activity. can anyone help me explain the meaning of the negative value...

17 February 2021 4,118 1 View

Has anyone performed a colorimetric RT-LAMP assay using Lucigens LavaLAMP kit?

Hi there, my lab recently opted to use LavaLAMP from Lucigen instead of the Warmstart kit from NEB. However, Lucigen has yet to create a colormetric kit for RT-LAMP, and adding phenol-Red (pH...

17 February 2021 5,399 1 View

How many of absolute errors (in kcal) do you believe affect the prescription of the diet when estimating the energy required for an elite athlete?

Dear colleagues, I am writing an article that compares the measured and predicted resting metabolic rate in elite athletes. However, to define a cutoff point to determine which equation is more...

16 February 2021 4,515 3 View

What is the actual chemistry going on between iodate and ascorbic acid?

I am studying biochemistry at university, as part of the course we do several titration experiments. In our handouts, we were given the following half equations: IO3- + 6H+ + 4e- I+ +...

16 February 2021 8,731 1 View