I want to synthesis phospho lactate. Please suggest a protocol which is specific for lactic acid or in general for any hydroxy acid? this involves phosphorylation of the OH group. thanks in advance
Are there any vendors for the following chemicals in INDIA other than SIGMA Dibenzyl N,N-diisopropylphosphoramidite Tetrol-7 thanks in advance
09 October 2018 8,220 0 View
I am working on a mesophilic protein (37 kDa and dimer in solution). I wanted to crystalize this protein and hence concentrated it to ~10 mg/mL. when kept on ice, the protein precipitated. kept...
07 August 2018 9,826 6 View
We wanted to tag a protein of interest with GFP mut3 version. upon generating the construct and sequencing it we found it has an additional mutation. A conserved leucine at 60th position had got...
07 August 2018 7,724 0 View
I am trying to crystallize a mesophilic enzyme and had set up screening for conditions by microbatch method. I saw huge structures in many conditions (images attached) after 3 weeks. But when we...
07 August 2018 7,475 0 View
Hi, I am interested in nucleotide metabolism and performed serial dilution and spotting using ade1 and ade2 knockout yeast strains. it seems like ade1 grows slower than ade2 eventhough they are...
01 February 2018 9,160 0 View
I am planning to generate a Auxin induced degron parasite (P. berghei) for a gene of interest. literature is available for only ex vivo studies in the presence and absence of auxin. Is it possible...
31 December 2017 7,790 0 View
Hi, I am trying to secrete a heterologous protein in S. cerevisiae using alpha mating factor signal peptide at the N-terminus and GFP at c-terminus. The plasmid is a 2 micron (Ura selection)...
08 September 2017 7,037 7 View
I did a blast search with a sequence of my protein of interest. I got around 4000 sequences in the result. How do I eliminate redundant sequences and multiple entries for the same organism from a...
05 June 2017 4,309 4 View
Hi, I am working with a yeast strain which is knockout for a gene of interest (AMP Deaminase) (gene replaced by g418 casette). this strain cannot grow on S-adenosyl methionine (SAM). I transformed...
01 February 2016 4,650 2 View
In serial dilution and spotting assay using yeast (for any particular growth defect phenotype) we see that, at times there is considerable growth at lower dilutions (high cell density) and growth...
31 December 2015 9,970 3 View
Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.
07 August 2024 8,782 3 View
Palmitic acid presence in aqueous fraction
05 August 2024 8,624 4 View
Hello, I am currently analyzing some phosphoproteomics data, but I have peptides with multiple phosphorylation sites or phosphorylations together with carbamidomethylation or oxidation. How can I...
04 August 2024 8,432 3 View
I used humic acid at 0.044 g/kg soil in my pot experiment. But finally, I have to recommend kg/ha. Each pot's soil weight was 11 kg. What is the solution?
02 August 2024 7,186 6 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
I would like to know the role of the TFA (trifluoroacetic acid) used on reverse phase chromatography c-18 column. It's is often used with a concentration of 0.1% on the mobile phase.
23 July 2024 8,075 3 View
Thanks
22 July 2024 1,143 3 View
Suggest the best and shortest route for the chemical synthesis and and green synthesis of magnetite(Fe3O4) The chemical synthesis should contain the least dangerous chemicals along with full...
21 July 2024 2,476 2 View
As mentioned in literature the addition of CaCO3 to MRS Agar media is considered as an indicator for lactic acid producing bacteria, so I wonder if we can add CaCO3 calcium carbonate to any other...
20 July 2024 7,504 0 View
I synthesised a nanocarrier by conjugation MOF (MIL-100) with folic acid for use in chemotherapy but the samples didn't dissolve completely in aqueous solution or DMSO. Instead, they only...
19 July 2024 8,522 0 View