Where can I find the following reagents?

Are there any vendors for the following chemicals in INDIA other than SIGMA Dibenzyl N,N-diisopropylphosphoramidite Tetrol-7 thanks in advance

09 October 2018 8,136 0 View

Reversible temperature dependent protein precipitation ?

I am working on a mesophilic protein (37 kDa and dimer in solution). I wanted to crystalize this protein and hence concentrated it to ~10 mg/mL. when kept on ice, the protein precipitated. kept...

07 August 2018 9,752 6 View

Leucine 60 to proline mutation in GFPmut3?

We wanted to tag a protein of interest with GFP mut3 version. upon generating the construct and sequencing it we found it has an additional mutation. A conserved leucine at 60th position had got...

07 August 2018 7,631 0 View

Soft and gel like protien crystals?

I am trying to crystallize a mesophilic enzyme and had set up screening for conditions by microbatch method. I saw huge structures in many conditions (images attached) after 3 weeks. But when we...

07 August 2018 7,397 0 View

Is there growth difference between ade1 and ade2 knockout yeast?

Hi, I am interested in nucleotide metabolism and performed serial dilution and spotting using ade1 and ade2 knockout yeast strains. it seems like ade1 grows slower than ade2 eventhough they are...

01 February 2018 9,067 0 View

Can in vivo studies be performed on Auxin induced degron P. berghei parasites in mice?

I am planning to generate a Auxin induced degron parasite (P. berghei) for a gene of interest. literature is available for only ex vivo studies in the presence and absence of auxin. Is it possible...

31 December 2017 7,719 0 View

"yeast secretory expression of heterologus proteins"?

08 September 2017 6,975 7 View

Generation of sequence alignment and Phylogenetic tree (for protein)

I did a blast search with a sequence of my protein of interest. I got around 4000 sequences in the result. How do I eliminate redundant sequences and multiple entries for the same organism from a...

05 June 2017 4,205 4 View

What is the probability of getting revertants after yeast transformation?

Hi, I am working with a yeast strain which is knockout for a gene of interest (AMP Deaminase) (gene replaced by g418 casette). this strain cannot grow on S-adenosyl methionine (SAM). I transformed...

01 February 2016 4,567 2 View

Query regarding serial dilution and spotting assay????

31 December 2015 9,880 3 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

Can I use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography?

Hi! I would want to know if I can use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography. This column has a polyethylene glycol phase, but I can't find the manual to check...

22 February 2021 730 2 View

Can you perform the Murexide test on samples fixed in Formalin?

I have a fixed sample and would like to use the murexide reaction to test for uric acid composition. I no longer have fresh samples

22 February 2021 1,328 1 View

Is there an ideal incubation time of RGO-modified SPCE before subsequent use, i.e., electrochemical characterization or further modification?

I notice that upon washing and air-drying of my RGO/SPCE surface and characterizing again via DPV, the current response towards ferricyanide probe significantly decreases. My worry is that the...

22 February 2021 5,324 3 View

I see 2 random bands in my ERK/pERK blot? Has anyone else seen this?

I ran a western blot using 12% gel and probing for ERK/pERK (cell signaling antibodies). The green is total ERK, the Red is supposed to be phospho-ERK. I see 2 bands below the total ERK that...

22 February 2021 1,777 3 View

What is the best etching method for Mg-Ca alloy?

I have used Picral etchant (4.2 g Picric acid, 10 mL Acetic acid, 70 mL Ethanol, 10 mL distilled water) but it didn't work. Is there any alternative?

21 February 2021 2,558 5 View