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Questions related to Optics
Hello I'm wondering if you have any resources or suggestions on how I can calculate the protein concentration from VP (viral particles) for adenoviruses? Thank you
25 July 2025 7,061 0 View
Hello. I am working with a recombinant enzyme from a marine bacterium, attempting to overexpress it in E. coli and purify it using His-tag affinity chromatography. Since I cannot perform all...
01 April 2025 2,399 12 View
What can i do whith negative ppm, i take it as they are or i shift all peaks to positive part, and what is about the aromatic peaks?
15 March 2025 6,476 1 View
Hello, How would I i design a accuracy study for dynamic light scattering? With a bead control and do different dilutions, and then calculate the % recovery? I would appreciate if you can...
25 February 2025 1,020 4 View
When I heat my protein in the presence of salt (~1 - 2 M) I observe some precipitate. Is there a way to analyse this precipitate? It is hardly soluble in any aqueous buffer or water itself. How do...
17 February 2025 1,898 0 View
Hello, I have been seeing a buffer peak that also appear in a sample peak. Ideally, it should not. So a particle is forming, and I see that in the sample peak, similar to what I see in the buffer...
14 February 2025 2,982 0 View
Hello, I would like to add a 3xHis residues to the N-terminal of a bacterial gene to clone and express, do I need to remove the starting codon from my gene of interest (GTG) in the forward primer...
18 January 2025 8,489 1 View
I need it for the DLS characterization
13 January 2025 1,439 1 View
Hello, I just want to know your thoughts of using the terms "capsid particles" and viral particles. I feel most people use these two terms interchangeably. They are not the same thing right?...
12 December 2024 7,874 3 View
Hello everyone, I am currently working on molecular cloning. After preparing the ligation mixture, I transformed it into cells. The next day, I observed colonies on LB medium with kanamycin,...
30 November 2024 3,675 6 View
I design a metasurface and when I try to run full structure simulation, I receive these errors. Please help me to fix it.
28 August 2024 6,514 0 View
If I want to improve the photocatalytic activity of Ni doped ZnO thin film then which would be the better deposition process for thin film Spin Coating or Dip Coating?
19 June 2024 7,811 2 View
Hello everyone, without going too much into details, my thesis is about improving the bonding strength at the wire bonding process. I should focus on laser pretreatment of the substrate. Has...
18 June 2024 469 1 View
What if we have bottom emissive OLED and the top-emissive microcavtiy OLED, which one will have a longer lifetime referring to the TRPL measurement?
25 May 2024 3,676 0 View
To get a unit cell's RCS and derive its S parameters from the RCS
13 February 2024 774 0 View
I am working on modifying polymer membranes with surfactants to improve their pervaporation performance. I noticed that the glass transition temperature (Tg) of the membranes decreased as the...
17 January 2024 5,453 4 View
Hello all dear If you know any reference or website about this subject, please share it with me Thanks in advance
09 November 2023 3,314 4 View
I designed an mzi structure where the input is complete, but the output changes badly at the end of the coupling zone.How do I need to change the structure？
27 October 2023 575 0 View
Cell line studies
24 October 2023 5,211 0 View
I am facing an issue in electroplating of copper, Nickel and Gold over the sputtered thin film of Titanium and Copper on Alumina substrate. We are depositing 60nm Titanium and 400nm of copper. Our...
05 October 2023 3,859 2 View
Why is (BER) in the near distance its value (1e-54) and in the far distance its value is (1e-9)? Is there a law for these calculations, noting that I worked (simulation) on the (Optisystem software) ?
27 September 2023 3,405 1 View
Hi everyone, in Rsoft there are choose to select ridge waveguide, However the profile index is step ( 90 degree-angle ) so, i want to create ridge waveguide at 70 degree . could you please guide...
08 September 2023 1,303 1 View
This is my new area of research who can help with video tutorials on the material geometry, layers, material sweep, wave equation, absorbance etc.
25 July 2023 367 0 View
At present a large number of mirrors are placed around the receiver and concentrate all of them on the receiver.why can't we use convex lens to concentrate sunlight
15 July 2023 1,576 1 View