Synthesis of phosphorylated hydroxy acids?

I want to synthesis phospho lactate. Please suggest a protocol which is specific for lactic acid or in general for any hydroxy acid? this involves phosphorylation of the OH group. thanks in advance

09 October 2018 2,619 0 View

Reversible temperature dependent protein precipitation ?

I am working on a mesophilic protein (37 kDa and dimer in solution). I wanted to crystalize this protein and hence concentrated it to ~10 mg/mL. when kept on ice, the protein precipitated. kept...

07 August 2018 9,752 6 View

Leucine 60 to proline mutation in GFPmut3?

We wanted to tag a protein of interest with GFP mut3 version. upon generating the construct and sequencing it we found it has an additional mutation. A conserved leucine at 60th position had got...

07 August 2018 7,631 0 View

Soft and gel like protien crystals?

I am trying to crystallize a mesophilic enzyme and had set up screening for conditions by microbatch method. I saw huge structures in many conditions (images attached) after 3 weeks. But when we...

07 August 2018 7,397 0 View

Is there growth difference between ade1 and ade2 knockout yeast?

Hi, I am interested in nucleotide metabolism and performed serial dilution and spotting using ade1 and ade2 knockout yeast strains. it seems like ade1 grows slower than ade2 eventhough they are...

01 February 2018 9,067 0 View

Can in vivo studies be performed on Auxin induced degron P. berghei parasites in mice?

I am planning to generate a Auxin induced degron parasite (P. berghei) for a gene of interest. literature is available for only ex vivo studies in the presence and absence of auxin. Is it possible...

31 December 2017 7,719 0 View

"yeast secretory expression of heterologus proteins"?

08 September 2017 6,975 7 View

Generation of sequence alignment and Phylogenetic tree (for protein)

I did a blast search with a sequence of my protein of interest. I got around 4000 sequences in the result. How do I eliminate redundant sequences and multiple entries for the same organism from a...

05 June 2017 4,205 4 View

What is the probability of getting revertants after yeast transformation?

Hi, I am working with a yeast strain which is knockout for a gene of interest (AMP Deaminase) (gene replaced by g418 casette). this strain cannot grow on S-adenosyl methionine (SAM). I transformed...

01 February 2016 4,567 2 View

Query regarding serial dilution and spotting assay????

31 December 2015 9,880 3 View

Is there any published or unpublished statistics on dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, US, UK or any other country?

Are there any published or unpublished statistics or papers on the dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, USA, UK, or any other country where vaccination has started at...

28 February 2021 4,237 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View

Anybody write me the name of scopus journals in commerce, management, economics areas. Which publish without any charge?

Please write those scopus journal names for which you have good experience with them.

24 February 2021 6,210 1 View

What does it require to be ready for e-Commerce in developing countries?

Most developing countries are not ready for e-Commerce? What dimensions and indicators are there to measure e-commerce readiness of a nation? Please share framework or model and evaluation...

22 February 2021 8,132 6 View

HPLC baseline failure (ACN/Water)?

My baseline increases without any explanation, it is only fixed by changing the solution B, will it be the column? Reagents? Bomb? Detector? Solution A: H3PO4 0.01% IN WATER Solution B: H3PO4...

21 February 2021 5,656 3 View

Gallium Antimonide (GaSb). Can anyone help me to find vendor (provider) to sell GaSb solar cell ?

I am searching of provider (GaSb), I only found this one (https://jxcrystals.com/wp/about/). Can anyone help me?

21 February 2021 1,945 2 View

Which transfection reagent is the best for plasmid transfection of MDA-MB-231 cell line, Lipofectamine based or other reagent suggestion?

I try to transfect MDA MB 231 cell line with a plasmid by PEI, unfortunately, the rate of transfection is low, and now I wanna find and order the best transfection reagent for this cell line and...

19 February 2021 828 6 View

Please suggest the website or link for the collection of data for (Cancer Incidance Rates in India)?

We are doing research for the prediction of cancer incidance rates increases in India upto next five years. Please help us to get the exact data for Cancer Incidance Rates in India. This will...

19 February 2021 8,478 2 View

Any information about dinitrosalisylic acid reagent end point color stability ?

I work on estimation of alpha amylase activity using dinitrosalisylic acid reagent If i put the reagent to stope the enzymatic reaction and allow the reagent to react with the produced reducing...

17 February 2021 7,402 4 View