I am planning to generate a Auxin induced degron parasite (P. berghei) for a gene of interest. literature is available for only ex vivo studies in the presence and absence of auxin. Is it possible to do the same invivo by feeding Auxin to the mouse?
Are there any vendors for the following chemicals in INDIA other than SIGMA Dibenzyl N,N-diisopropylphosphoramidite Tetrol-7 thanks in advance
09 October 2018 8,136 0 View
I want to synthesis phospho lactate. Please suggest a protocol which is specific for lactic acid or in general for any hydroxy acid? this involves phosphorylation of the OH group. thanks in advance
09 October 2018 2,619 0 View
I am working on a mesophilic protein (37 kDa and dimer in solution). I wanted to crystalize this protein and hence concentrated it to ~10 mg/mL. when kept on ice, the protein precipitated. kept...
07 August 2018 9,752 6 View
We wanted to tag a protein of interest with GFP mut3 version. upon generating the construct and sequencing it we found it has an additional mutation. A conserved leucine at 60th position had got...
07 August 2018 7,631 0 View
I am trying to crystallize a mesophilic enzyme and had set up screening for conditions by microbatch method. I saw huge structures in many conditions (images attached) after 3 weeks. But when we...
07 August 2018 7,397 0 View
Hi, I am interested in nucleotide metabolism and performed serial dilution and spotting using ade1 and ade2 knockout yeast strains. it seems like ade1 grows slower than ade2 eventhough they are...
01 February 2018 9,067 0 View
08 September 2017 6,975 7 View
I did a blast search with a sequence of my protein of interest. I got around 4000 sequences in the result. How do I eliminate redundant sequences and multiple entries for the same organism from a...
05 June 2017 4,205 4 View
Hi, I am working with a yeast strain which is knockout for a gene of interest (AMP Deaminase) (gene replaced by g418 casette). this strain cannot grow on S-adenosyl methionine (SAM). I transformed...
01 February 2016 4,567 2 View
31 December 2015 9,880 3 View
Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?
03 March 2021 1,763 2 View
Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,
02 March 2021 0 0 View
I have designed reflectarray element to generate OAM beams at 5.8GHz and feeding it though Horn Antenna on HFSS. After full wave simulation, i am not getting desired results i.e Radiation pattern...
01 March 2021 9,701 5 View
Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...
01 March 2021 6,953 1 View
Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...
01 March 2021 9,938 3 View
Hi dear researchers I connected a rigid body into the part of my structure by tie constraint and then I added displacement to rigid body, in static general step every thing is fine and these two...
28 February 2021 3,442 2 View
I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....
28 February 2021 7,967 3 View
Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!
28 February 2021 3,951 2 View
Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...
25 February 2021 155 2 View
Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...
24 February 2021 1,825 3 View