I m doing research on biofuel production from lignocellulosic biofuel. I have Cellulase Enzyme from Aspergillus niger (Sigma), is this sufficient for the hydrolysis process? If anyone knows the detailed protocol about enzyme used method please recommend me for the process.
Thanks in advance!
״Sufficient" in what way?
I suggest you read some of the vast literature on the subject. Things like which biomass, what preatreatment etc could make a huge different.
Forgot to mention earlier, most likely you will also need cellobiase, as the product of cellulose breakdown by cellulase is cellobiose and not glucose, and (most) yeast can’t utlise cellobiose.
Respected Yoram Gerchman, Thank you for your answer. I will do research on that Cellulase product which can break all the Glycosidic bond or not and get back to you soon.
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