13 Questions 303 Answers 0 Followers
Questions related from Yoram Gerchman
Greetings We are trying to study Cholesteryl esters. We start with MeOH/Chloroform extraction and then remove most of the noise by silica column, but the Cholesteryl esters come out with...
03 June 2024 5,484 0 View
Greetings I am working with a plasmid encoding the restriction enzyme EcoRV. Do you know of E. coli strain resistance to EcoRV restriction?
07 January 2024 8,171 4 View
Greetings all. As you probably know the "Tryptone" used as component of LB media and alike are the product of digestion of casein. Similarly Cas-Amino acids are the product of acidic hydrolysis of...
10 January 2022 1,953 1 View
Greetings all I am looking for a way to search for specific gene(s) expression levels in an environmental transcriptome data posted on MG-RAST. If anyone has expertise in doing such it would be...
10 May 2020 2,526 0 View
Greetings We want to look at the effect of Stratum Corneum composition on bacterial attachment. Does anyone know a commercially available "stratum corneum substitute" lipid mixture? Or is willing...
18 November 2019 1,489 2 View
Greetings I am looking for a trusted protocol for extraction of Heme A, Heme B and Heme O, to be used as standards in HPLC analysis. Alternatively if you know of a commercial source also...
28 May 2019 5,340 2 View
Greetings I am trying to find out what is the origin of the 220nm absorbance peak of free amino acids? It is usually attributed to peptide bond but I find it also in single aa... Yoram
18 June 2018 5,730 3 View
Greetings all I am looking for a fluorophore with excitation at the UVC range (250~260nm) and emission in the UVA/visible range. Ideally one that will not bleach easily. Many thanks Yoram
06 March 2018 1,488 0 View
Greetings I’m looking for numbers (rather then general statments) on the energetic cost of different biomass pretreatments before enzyme digestion for sugar release. I am especialy intrested in...
29 January 2018 5,768 5 View
Greetings We need to buy an RI detector for our Agilent 1200 HPLC system (connected to a computer running ChemStation). Given the high price of new detector I thought about buying a used one....
20 October 2017 6,386 9 View
Greetings We need to create a 3D structure that will be used inside a microwave (and will contain plant biomass suspended in water). What polymer (e.g. ABS, nylon, other) would be microwave...
17 May 2017 5,168 2 View
Greetings Does anyone know the history of E. coli XL10-gold (strategene)? Did it originate from XL1-Blue and what was the procedure? If you have a reference even better. Yoram
19 October 2016 3,959 2 View
Greetings. I am looking for Q-Dots or other high yield fluorophore that exite at ~254nm and emit at ~300nm. Any ideas welcome Yoram
28 March 2016 9,428 2 View
