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Questions related from Yoram Gerchman
Greetings all I am trying to compare two closely related bacterial genomes (two strains of E. coli) and get a list of the differences. Not the similarities but the differences. Any idea for a web...
27 May 2025 4,879 4 View
Greetings We are trying to study Cholesteryl esters. We start with MeOH/Chloroform extraction and then remove most of the noise by silica column, but the Cholesteryl esters come out with...
03 June 2024 5,592 0 View
Greetings I am working with a plasmid encoding the restriction enzyme EcoRV. Do you know of E. coli strain resistance to EcoRV restriction?
07 January 2024 8,259 4 View
Greetings all. As you probably know the "Tryptone" used as component of LB media and alike are the product of digestion of casein. Similarly Cas-Amino acids are the product of acidic hydrolysis of...
10 January 2022 2,034 1 View
Greetings all I am looking for a way to search for specific gene(s) expression levels in an environmental transcriptome data posted on MG-RAST. If anyone has expertise in doing such it would be...
10 May 2020 2,618 0 View
Greetings We want to look at the effect of Stratum Corneum composition on bacterial attachment. Does anyone know a commercially available "stratum corneum substitute" lipid mixture? Or is willing...
18 November 2019 1,576 2 View
Greetings I am looking for a trusted protocol for extraction of Heme A, Heme B and Heme O, to be used as standards in HPLC analysis. Alternatively if you know of a commercial source also...
28 May 2019 5,430 2 View
Greetings I am trying to find out what is the origin of the 220nm absorbance peak of free amino acids? It is usually attributed to peptide bond but I find it also in single aa... Yoram
18 June 2018 5,815 3 View
Greetings all I am looking for a fluorophore with excitation at the UVC range (250~260nm) and emission in the UVA/visible range. Ideally one that will not bleach easily. Many thanks Yoram
06 March 2018 1,560 0 View
Greetings I’m looking for numbers (rather then general statments) on the energetic cost of different biomass pretreatments before enzyme digestion for sugar release. I am especialy intrested in...
29 January 2018 5,845 5 View
Greetings I am trying to find out what is the natural habitat of Synechocystis sp. PCC 6803 and Nostoc PCC 7120. Many thanks Yoram
11 December 2017 420 3 View
Greetings We need to buy an RI detector for our Agilent 1200 HPLC system (connected to a computer running ChemStation). Given the high price of new detector I thought about buying a used one....
20 October 2017 6,493 9 View
Greetings We need to create a 3D structure that will be used inside a microwave (and will contain plant biomass suspended in water). What polymer (e.g. ABS, nylon, other) would be microwave...
17 May 2017 5,255 2 View
Greetings Does anyone know the history of E. coli XL10-gold (strategene)? Did it originate from XL1-Blue and what was the procedure? If you have a reference even better. Yoram
19 October 2016 4,033 2 View
Greetings. I am looking for Q-Dots or other high yield fluorophore that exite at ~254nm and emit at ~300nm. Any ideas welcome Yoram
28 March 2016 9,497 2 View
Greetings all Has anyone tried or know of a reference to using Rhodamin B/olive oil assay to test lipase activity in liquid (rather then in agar)? Many thanks Yoram
19 January 2015 9,816 3 View
