Ganesh Lamichhane

4 Questions 9 Answers 0 Followers

Questions related from Ganesh Lamichhane

Can we measure amount of peptide after BSA protein digestion by using BCA peptide assay?

Hello, everyone!! I've included a general question above, and the answer is yes. However, I am discussing SP3 approaches. I've noticed that several authors haven't discussed peptide recovery...

05 December 2024 1,158 2 View

How can we find different conformational structure of protein?

Dear Researcher, I am using PyMOL software to visualize the protein structure. I would like to make open/closed/helix or different conformational changes in protein during the catalytic...

12 September 2022 2,974 4 View

Is there commercial cellulase enzyme alone sufficient for cellulose hydrolysis for biofuel production?

I m doing research on biofuel production from lignocellulosic biofuel. I have Cellulase Enzyme from Aspergillus niger (Sigma), is this sufficient for the hydrolysis process? If anyone knows the...

25 November 2019 9,278 3 View

Trypsin Digestion and Peptide assay.

Dear Researchers, I have recently encountered with BCA peptide assay after Trypsin digestion. I have to quantify the 35ng/uL of protein after digestion of 35ng/uL of BSA. After several times, I...

01 January 1970 3,372 4 View