Has anyone experience about isolation of cancer stem cells from cancer cell lines?

I am trying to isolate breast cancer stem cells from breast cancer cell lines through mammosphere formation. I see perfect spheres under microscope but all of them are CD24+/CD44- and dont show...

11 December 2016 4,216 6 View

How can i dilute 35 microgram bFGF/35 microliter Tris 5mM ?

Hi, i want to use 20ng/ml BFGF in culture media. How can i dilute 35 microgram bFGF/35 microliter Tris 5mM without any problem for breast cancer stem cells?

08 September 2016 2,029 1 View

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07 August 2016 6,051 3 View

Is it possible to use low attachment cell plates instead of ultra-low attachment plates?

Is it possible to use low attachment cell plates instead of ultra-low attachment plates for mammosphere formation with a same efficiency?

05 June 2016 8,617 3 View

How can I make a ultra-low attachment plates manually?

Read 3 answers by scientists with 2 recommendations from their colleagues to the question asked by Saghar Yousefnia on Jun 26, 2016

05 June 2016 6,296 3 View

What is the difference in quality between isolation of cancer stem cells from tissues and cell line?

Is there any difference in quality between isolation of cancer stem cells from tissues and cell line?can anyone tell me about advantages and disadvantages?

03 April 2016 2,603 0 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View