Is there anyone who has an experience about isolation of breast cancer stem cells from breast cancer cell line?

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Has anyone experience about isolation of cancer stem cells from cancer cell lines?

I am trying to isolate breast cancer stem cells from breast cancer cell lines through mammosphere formation. I see perfect spheres under microscope but all of them are CD24+/CD44- and dont show...

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Hi, i want to use 20ng/ml BFGF in culture media. How can i dilute 35 microgram bFGF/35 microliter Tris 5mM without any problem for breast cancer stem cells?

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Is it necessary to use β-Mercaptoethanol in MCF-7 culture media or other breast cancer cell lines?

Is it necessary to use β-Mercaptoethanol in MCF-7 culture media or other breast cancer cell lines? does it Interfere with the mammosphere formation?

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Is it possible to use low attachment cell plates instead of ultra-low attachment plates?

Is it possible to use low attachment cell plates instead of ultra-low attachment plates for mammosphere formation with a same efficiency?

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How can I make a ultra-low attachment plates manually?

Read 3 answers by scientists with 2 recommendations from their colleagues to the question asked by Saghar Yousefnia on Jun 26, 2016

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What is the difference in quality between isolation of cancer stem cells from tissues and cell line?

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Go J Yoshida

The following breast cancer cell lines can be raised as suitable for the isolation of cancer stem cells (CSCs); SKBR-3 (luminal), T47D (luminal)MDA-MB231 (basal). THe mot typical method for CSC isolation is the flow cytometry with CD24 and CD44 antibodies; CSCs exist in the CD44(high)/CD24(low/negative) subpopulation. You further better check the ALDH1 activity after isolation!!!

Saghar Yousefnia

Is there any difference in quality between isolation of cancer stem cells from tissues and cell line?advantages and disadvantages?