Saghar Yousefnia

7 Questions 6 Answers 0 Followers

Questions related from Saghar Yousefnia

Has anyone experience about isolation of cancer stem cells from cancer cell lines?

I am trying to isolate breast cancer stem cells from breast cancer cell lines through mammosphere formation. I see perfect spheres under microscope but all of them are CD24+/CD44- and dont show...

12 December 2016 4,268 6 View

How can i dilute 35 microgram bFGF/35 microliter Tris 5mM ?

Hi, i want to use 20ng/ml BFGF in culture media. How can i dilute 35 microgram bFGF/35 microliter Tris 5mM without any problem for breast cancer stem cells?

09 September 2016 2,121 1 View

Is it necessary to use β-Mercaptoethanol in MCF-7 culture media or other breast cancer cell lines?

Is it necessary to use β-Mercaptoethanol in MCF-7 culture media or other breast cancer cell lines? does it Interfere with the mammosphere formation?

08 August 2016 6,099 3 View

Is it possible to use low attachment cell plates instead of ultra-low attachment plates?

Is it possible to use low attachment cell plates instead of ultra-low attachment plates for mammosphere formation with a same efficiency?

06 June 2016 8,665 3 View

How can I make a ultra-low attachment plates manually?

Read 3 answers by scientists with 2 recommendations from their colleagues to the question asked by Saghar Yousefnia on Jun 26, 2016

06 June 2016 6,398 3 View

What is the difference in quality between isolation of cancer stem cells from tissues and cell line?

Is there any difference in quality between isolation of cancer stem cells from tissues and cell line?can anyone tell me about advantages and disadvantages?

04 April 2016 2,644 0 View

Is there anyone who has an experience about isolation of breast cancer stem cells from breast cancer cell line?

i want to isolate breast cancer stem cells from breast cancer cell line with purpose of drug researches. can anyone suggest me which cell line is better to isolate? is it possible for any kinds...

04 April 2016 8,556 2 View