Is there any difference in quality between isolation of cancer stem cells from tissues and cell line?can anyone tell me about advantages and disadvantages?
I am trying to isolate breast cancer stem cells from breast cancer cell lines through mammosphere formation. I see perfect spheres under microscope but all of them are CD24+/CD44- and dont show...
11 December 2016 4,320 6 View
Hi, i want to use 20ng/ml BFGF in culture media. How can i dilute 35 microgram bFGF/35 microliter Tris 5mM without any problem for breast cancer stem cells?
08 September 2016 2,155 1 View
Is it necessary to use β-Mercaptoethanol in MCF-7 culture media or other breast cancer cell lines? does it Interfere with the mammosphere formation?
07 August 2016 6,159 3 View
Is it possible to use low attachment cell plates instead of ultra-low attachment plates for mammosphere formation with a same efficiency?
05 June 2016 8,711 3 View
Read 3 answers by scientists with 2 recommendations from their colleagues to the question asked by Saghar Yousefnia on Jun 26, 2016
05 June 2016 6,419 3 View
i want to isolate breast cancer stem cells from breast cancer cell line with purpose of drug researches. can anyone suggest me which cell line is better to isolate? is it possible for any kinds...
03 April 2016 8,578 2 View
What are the emerging trends and opportunities for STEM education and careers in the online space?
10 August 2024 507 0 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...
06 August 2024 666 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View