Is there anyone who did the isolation of the apicoplast from Plasmodium falciparum?

How can i select suitable gene sequence at Ring stage of Plasmodium Falciparum for primer design?

I am new to PCR. We are working on PCR based amplification of targeted DNA sequence of Plasmodium Falciparum. For that, We are targeting the Ring stage of the parasite. I want to know on which...

15 December 2020 6,954 3 View

Can someone please share a protocol for the production of recombinant proteins in E.coli BL21 DE3? or the use of plasmid pET 23B?

this for obtein a protein of plasmodium please

24 April 2020 4,302 3 View

Where and how to get Research collaboration and PhD sandwich program?

I am currently carrying ou a study on Plasmodium faclciparum malaria and concomitant bacteria infection. I will need an expart to collaborate with especially in molecular epidemiology, and...

07 August 2019 9,877 1 View

How to calculate number of infected RBC in mice which is infected with plasmodium berghei?

In my experiment I have to infect 2*10^7 infected RBC equally to a group of mice equally by collecting blood from donor mice.How to calculate number of infected RBC?

04 May 2019 8,078 1 View

Can heparin tubes be used to collect blood samples for Plasmodium falciparum microscopy?

I need to collect blood samples for microscopy of P. falciparum. Which tube is best for this? Heparin or plain vacutainer tubes? Will RBC lysis affect detection of the parasite? Thank you.

23 April 2019 8,803 4 View

Is there a system to grow large numbers of Plasmodium gametocytes with reduced labour?

Hello! I am researching alternative methods to grow large numbers of Plasmodium falciparum gametocytes (for mosquito infections) which are not the traditional tissue culture flasks, and so can be...

21 April 2019 2,699 1 View

Is there a system to grow large numbers of Plasmodium gametocytes with reduced labour?

Hello! I am researching alternative methods to grow large numbers of Plasmodium falciparum gametocytes (for mosquito infections) which are not the traditional tissue culture flasks, and so can be...

03 April 2019 7,533 1 View

Is there any way to quantify the amount of merozoites of Plasmodium chabaudi other than by flow cytometry?

I'm doing an interaction test between erythrocytes of mice and free merozoites of Plasmodium chabaudi. Is there any way to quantify merozoites other than by flow cytometry? I have been trying in...

03 April 2019 9,682 3 View

Is it right to use DNA from Malaria Positive human blood sample a PCR positive control for Plasmodium from Wild e.g bat?

Good day house. Please I want to screen about 100 DNA samples extracted from bats' blood for Plasmodium and its relatives. For the PCR I need positive control. Is it right for me to extract DNA...

03 April 2019 7,585 5 View

Best protocol for mercurochrome staining of mosquito midguts?

I should isolate single Plasmodium falciparum oocysts from mosquito midguts. In order to do that, I have to stain the midguts first. My present protocol is the following 1. a drop of 1%...

17 March 2019 106 1 View