Anyone with lab experience with chromosomally tagged Pseudomonas aeruginosa analysis on FLOW CYTOMETRY?

17 October 2017 0 4K Report

I am working with clinical Pseudomonas aeruginosa isolates and i am straggling with getting clean population and enough population of cfp, yfp and gfp tagged Pa.

I am wondering if anyone has experience in running such samples so we can exchange some troubleshooting on the signal strength and the tagging stability.

thanks

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