Dear Academics,
I want to download whole genome sequence of falciparum isolates from different countries in PlasmoDB database? For instance, I want to whole geneome data of isolates from Nigeria, South Africa etc
Thanks
I want to run copy number analysis on falciparum isolates using relative quantification. However I do not understand the function and difference between housekeeping gene and positive control in...
22 May 2023 2,501 4 View
Dear Intellectuals, Does anyone know how to get the expected band size of parasites or Plasmodium to be precise? Thank you for your expected useful guidance.
29 May 2022 2,021 1 View
I was previously working with 1molar of primer working solution. But I ordered new set of primers from another company whose protocol came in picomoles. How do I prepare my normal 1 molar working...
20 March 2022 8,819 5 View
Body centered cubic
03 February 2022 9,877 0 View
Is it the responsibility of research supervisors to encourage his/her students or he/she should not show concern?
24 June 2020 5,473 55 View
Some of my data are not normally distributed with the kolmogorov-smirnov and Shapiro-wilk tests being significant with values of 0.00. Although Kurtosis and skewness were within -2 and +2, normal...
15 June 2020 1,022 6 View
In a study to determine the predictors of Gestational diabetes mellitus (GDM) in pregnancy, the diagnosis of GDM was used as the outcome variable ( Dependent variable). This was a dichotomous...
10 June 2020 8,654 3 View
Most studies that used the body mass index (BMI) as a measure of obesity in scientific researches did not classify BMI according to gender(male or female). Is there a need to control for gender...
06 June 2020 9,705 0 View
What is the best and reliable method for COVID-19 diagnosis?
05 May 2020 9,394 7 View
Hello everyone, I am currently rounding up my MEng programme. Based on my interests during my masters, I intend to focus my PhD at the intersection of PID control, information theory and...
24 March 2020 3,039 5 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
TEP presentation caption (The Environmental Project) Re: Why should Washington’s DC, or any country government point of location think of as nowadays of as to being 'tomorrow as to come! if it...
03 August 2024 2,484 1 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
Given that the bacterial genome has over 800 contigs, but its quality metrics are good, with a completeness of 98.55% and a contamination of 0.68% as assessed by CheckM, what specific validation...
01 August 2024 1,514 1 View
Hello everyone, I am currently looking for tools to recovery viral genomes from bacterial genomes, not metagenomes. However, I have only found tools that are designed for retrieving and studying...
28 July 2024 8,953 1 View
Hi all. As a beginner in ChIP-seq experiments, I hope you understand that the following questions might be somewhat basic. I am planning to perform ChIP-seq or MeDIP-seq analysis to investigate...
28 July 2024 6,938 1 View
Gene sequencing related trouble shooting
25 July 2024 4,149 2 View
Hi all, My lab has Thermo Scientific™ Invitrogen™ EVOS™ FL Auto 2 Imaging System, and I was wondering if I will be able to use it with whole blood, whilst focusing on platelets? The idea would...
24 July 2024 9,337 3 View
How the damages of building and shopping centers affected the south African economy?
24 July 2024 5,401 2 View