Problem in docking energy calculation using iGEMDOCK for high molecular weight protein?

I am using iGEMDOCK software to calculate the docking binding energy for my legand compound with protein 2cr0 but it shows error and says that molecular weight is high for this protein is there is...

31 December 2016 1,918 1 View

How could I make a hungtinton's disease in-vitro model using the SH-SY5Y cell line?

07 August 2016 5,755 1 View

What Is that problem when culture the hippocmapal cell in 12mm glass coverslip coaated with Poly D lysine?

I am doing culture of hippocampal cell in coverslip coated with 1mg Poly D lysine/ml 0.1M boric acid solution (PH 8.5) but now a days its not working fine as what could be the problem. Usually...

02 March 2016 6,866 4 View

Is that is possible to call sitgmasterol as a drug candidate for CNS?

31 December 2015 7,244 2 View

Which solvent con dissolve Stigmastrol (sigma) beside Chloroform ?

05 June 2015 5,766 7 View

How could i remove my neuronal cultue cell from coverslip for performing electrophysiology ?

During perfprmance of electrophysilogy on nuronal primery culture cell that has attached to the coveslip i could not patch it well and seal the patch also to hold for long time to measure the...

11 December 2014 9,720 8 View

Is anyone familiar with primary cultures of Substantia nigra of Rat brain?

11 December 2014 3,680 3 View

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02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View