Is anyone familiar with primary cultures of Substantia nigra of Rat brain?

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Katie Cheung

Agree with Adil. Immunostaining is a good way to identify a certain population of neurons, say dopaminergic neurons in substantia nigra.

Sachiko Murase

Dear Md,

Substantia nigra contains dopamine neurons, which can be identified by immunostaining against tyrosine hydoxylase (TH). See my J. Neurosci. paper (2006).

Cheers,

Sachiko

Md Nazmul Haque Habib

thanks all of you for your kind suggession