Problem in docking energy calculation using iGEMDOCK for high molecular weight protein?

I am using iGEMDOCK software to calculate the docking binding energy for my legand compound with protein 2cr0 but it shows error and says that molecular weight is high for this protein is there is...

31 December 2016 1,918 1 View

What Is that problem when culture the hippocmapal cell in 12mm glass coverslip coaated with Poly D lysine?

I am doing culture of hippocampal cell in coverslip coated with 1mg Poly D lysine/ml 0.1M boric acid solution (PH 8.5) but now a days its not working fine as what could be the problem. Usually...

02 March 2016 6,866 4 View

Is that is possible to call sitgmasterol as a drug candidate for CNS?

31 December 2015 7,244 2 View

Which solvent con dissolve Stigmastrol (sigma) beside Chloroform ?

05 June 2015 5,766 7 View

Is there any specific chemical to degenerate only the axon of a particular neuronal cell?

is there any specific chemical to degenerate only the axon of a particular neuronal cell

31 December 2014 1,105 2 View

How could i remove my neuronal cultue cell from coverslip for performing electrophysiology ?

During perfprmance of electrophysilogy on nuronal primery culture cell that has attached to the coveslip i could not patch it well and seal the patch also to hold for long time to measure the...

11 December 2014 9,720 8 View

Is anyone familiar with primary cultures of Substantia nigra of Rat brain?

11 December 2014 3,680 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

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02 March 2021 3,060 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View