How to extract RNA from brown adipose tissue(BAT)?

30 January 2021 6,610 3 View

How can we test interaction effect using repeated measure design?

Hi! I am currently working on a problem using repeated measure design. Each participant was assigned to two CONDITIONs (A and B). And their GOAL (continuous) were measured twice in condition A and...

25 November 2020 1,235 3 View

When performing mutliple behavioural tests over a period of time, should you habituate the animals before every test or just once at the beginning?

I am doing a baseline + 2 rounds of behavioural testing (NOR + NLR) - so total 3 rounds of testing, all 1 week apart in male SD rats. Is it necessary to habituate them before every test, or is...

17 June 2020 9,083 5 View

Can I use LS column and MidiMACS seperator from Miltenyi Biotec Neutrophil Isolation Kit for mouse peripheral blood?

We are using the neutrophil isolation kit from Miltenyi Biotec to get the neutrophils from peripheral blood from JAK2 constitutively active mice. This mouse is a leukemia model, so the traditional...

11 March 2020 8,611 3 View

Can I use satellite images to detect the leaking and clogging for a Surface Drip Irrigation System?

Hello everyone: I have being searching for three weeks now to find a possible solution using remote sensing. This application is for a vineyard 200m by 300m or even bigger. It has a a surface...

28 February 2020 3,723 5 View

Is it normal that Streptomyces fradiae culture is clumpy/particles/precipitated?

Hello, I cultured Streptomyces fradiae from a freeze-dried sample following ATCC protocol: use 6ml broth (tryptone 5g/l, yeast extract 3g/l) to rehydrate the sample and culture at 26 degrees...

05 February 2020 1,232 3 View

How to check the quality of MgO for magnetic tunnel junction?

I am making some magnetic tunnel junctions, but I always get the junction shorted. I am not sure whether the quality of MgO is good. Does anyone know how to check the quality of MgO? Such as AFM...

28 January 2020 139 2 View

Implement Topshim (Bruker NMR) shimming commands in IconNMR?

With Bruker's Topshim program, I have used the following command to shim every new sample: topshim z6 1d optsolvent coil=1H tuneb tunea Now I'd like to use IconNMR to automate this procedure,...

20 August 2019 1,111 5 View

How to dissolve and inject 4-hydroxytamoxifen to nude mouse?

What is the normal way to inject 500 ug 4-hydroxytamoxifen per mouse? Is it dissolved 4-OHT in ethanol and diluted with peanut oil at least 10 times?

10 May 2019 3,301 1 View

How to mesh in crack tip using shell element in ABAQUS?

S8R elements were applied in 2D cracked plate in ABAQUS. But when I drew a circle contour in the crack tip, it showed an error. I also checked the ANAQUS Manual and it told me that 'Focused...

21 April 2019 3,698 0 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View