Xiarong Shi

4 Questions 4 Answers 0 Followers

Questions related from Xiarong Shi

What is the best practice in propagating ITR-containing AAV transfer vector ?

I heard a lot of examples of troublesome ITR deletions in AAV vector propagation in bacteria. In general, people recommend using lower temperature (30oC) for culturing the bacteria or use some...

30 January 2020 7,099 0 View

What is the human U6 promoter transcription start site?

Many vectors expressing shRNA or microRNA bear the U6 promoter, but what is the exact transcription start site? I have heard people put an additional G before their small RNA insert, is this G the...

03 April 2014 5,964 3 View

Is there any shRNA expression before lentiviral integration into the host cell genome?

We are using lentiviral system to introduce shRNA expression into host cell. After viral infection, we use puromycin to select for integration for 48 hours. My question is, after the the viruses...

05 July 2013 5,618 2 View

What kind of phenotype is this?

When I over expressed an E3 ligase Cbl mutant in a 3T3 cell line, the cell morphology changed. I have repeated this several times. Is this related to transformation? The cells are selected for...

13 March 2013 4,781 18 View