The high rate of proliferation required of stem cells could produce genetic instability which may result in sporadic cancerous cells. Is there any research on this?
High rate of proliferation increases the risk of mutations. However, what makes you think that stem cells are highly proliferative?
I am looking to predict some production for future onshore wind energy production in Portugal, is there any database with info of performances of the existing wind farms in the country? something...
28 July 2024 1,750 1 View
hi, I am doing a risk analysis of a cylindrical fuselage with windows. I have applied contact interactions between the different parts, and I have applied a unit value load to a reference point,...
20 July 2024 647 2 View
In most of the literature I am reading at the moment, the abbreviation μg m−2 h−1 is used to express mean annual fluxes of greenhouse gases. I am confused by the "h" and supposed it stands for...
27 June 2024 9,633 1 View
Good day! I am currently testing my sample for MIC, may you help me how to interpret the results at OD630? how would I know if there is growth or not based on the values given by the microplate...
01 June 2024 7,920 3 View
The as the solution were mixed together, there was no color change, and then the UV-vis was read every 10min but barely any absorbance was observed. The solutions were 2,1mM of NaBH4 and 0,07g...
20 May 2024 615 1 View
How are industrial design research designs generally configured? What methodologies do they generally use? The idea is to have on hand a repertoire of methods and frequent actions for research in...
08 May 2024 7,666 2 View
I have two antibodies that are staining well in IHC but not in IF for tissue sections, when using the same concentration and antigen retrieval method for both. I also know the secondary works well.
08 May 2024 3,890 4 View
Hello, I am making a model of an airplane fuselage, consisting of the fuselage, frames and stringers. I have made “tie” joints between the different fuselage parts, and between the frames and...
25 April 2024 8,908 1 View
Hello! I have tried to electroporate the Jurkat cell line with the Neon System using the parameters provided by the supplier, but almost all the cells die after the procedure. I have also tried...
10 April 2024 1,382 0 View
sobre los principios del contractualismo social realizar un argumento
03 April 2024 4,585 0 View
What are the emerging trends and opportunities for STEM education and careers in the online space?
10 August 2024 507 0 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
what are the top 3 challenges to the advancement of the field of Radiogenomics in cancer research? is it the availability of easily available low-cost matched imaging and biosamples with clinical...
03 August 2024 5,828 4 View
I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...
01 August 2024 7,677 2 View
What types of mask designs for metal deposition, to be used in a PVD system, are best suited for perovskite-based solar cells?
31 July 2024 4,835 3 View
Hi there! I recently started working on Lx-2 cells. When I thaw the cells, they start attaching within few hours but later in about 2 days they started rounding and dying. I used DMEM+2% FBS...
31 July 2024 3,893 0 View