For my research, I would like to extract glucose, sucrose, and fructose from the crowns of strawberry plants and quantify these sugars using LC-MS. However, I have not had much luck finding a decent extraction method in the literature. Are there any links to a suitable simple sugar extraction method for solid plant material to be used with LC-MS?
Dear Angela,
https://www.derpharmachemica.com/pharma-chemica/liquid-chromatographymass-spectrometry-lcms-method-for-the--determination-of-sugars-in-fresh-pomegranate-fruit-juices.pdf
If paper will not be useful then references should be ...
https://iopscience.iop.org/article/10.1088/1742-6596/1065/23/232004/pdf
Article Quantification by LC-MS/MS of individual sugars in fruit jui...
Article Simultaneous determination of rhamnose, xylitol, arabitol, f...
Maybe references in these publications will be helpful,
Best regards
Tomasz
You can check these extraction techiques; QuEChERS and matrix solid-phase dispersion. These are used for mostly pesticide analysis in fruits and I am not sure if the methods are sutiable for your analytes. I couldn't see any article which utilizes MSPD to quantify your analytes but you can do further research. If there is no such study and it works, yours can be novel by that aspect.
Regards.
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