22 February 2021 2 8K Report

Hello!

I am interested in determining whether a poly-unsaturated fatty acid I have engineered E. coli to produce is being incorporated into the membrane of the cells or if it is simply a free fatty acid. Would anybody be able to provide me with a protocol to separate the bacterial membrane from the cytosol? I would like to extract the membranes selectively and then do GCMS to confirm the presence of the poly-olefin in the membrane.

Best,

Aidan

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