Does anyone know if the anti CD25 clone 3G10 cross reacts with feline CD25?

We are conducting FIV study and would like to stain feline PBMC's for CD25. One vendor calls the 3G10 clone "pan mammalian" but doesn't include feline in the list of known cross reactive species....

27 January 2020 8,961 1 View

Using Chromotek GFP-Trap Magnetic Agarose beads for Nuclear Protein Purification?

Hi All, I was wondering if anyone had used the chormotek gfp trap magenetic agarose bead kit for purification of GFP tagged nuclear proteins. I am attempting to purify a gfp-tagged protein...

14 January 2020 4,572 2 View

Is the concept of an addictive personality a myth?

I read the relevant wikipedia page and it seems quite intuitive as a construct, but I know that just because something seems to make sense doesn't make it true. Thoughts?

03 August 2019 3,467 4 View

Solutions to selectively break down proteins and leaving polymer chains intact?

In a project, I am required to degrade proteins to a size of 10Kd. This would prevent protein from passing through ultrafiltration and being absent in the resultant. I suggested using SDS,...

27 January 2019 3,300 3 View

Western Blot Signal Looks Like Backround Color?

I am designing an antibody to detect the protein LynA in western blot. Right now I have 8 potential antibodies (SCFVs) that may or may not bind by western blot. I have an apparatus that lets me...

28 November 2018 5,889 1 View

Taqman microRNA Assay Primer dimerisation at exceptionally high levels?

I have been using taqman microRNA assays for 3 years and have recently come across a very serious problem with one particular microRNA. RT-qPCR analysis shows the miRNA to be expressed at higher...

22 November 2018 8,147 3 View

Can I stain intestines with cresyl violet?

Hi everyone, I apologize if this has been asked before. I am running a study with rats and want to collect intestinal samples to look at histologically. Firstly, do the intestines need to be...

18 October 2018 6,288 2 View

Does the populations of argonaute-2 microRNAs and RISC loaded miRNA correlate with total miRNA within cells/tissue?

We've performed Profiling of Ago-2 RIP miRNA in tissue and also followed it with individual RT-qPCRs and showed that the miRNA levels between the 2 were correlated but this is with a handul of...

02 February 2018 1,841 2 View

Can mice reproduce after in utero injections?

I am thinking of doing in utero injections of mice to study the effects of a drug on the embryos. However, we hope to use the female mice again after it gives birth to the injected pups. Is it...

02 January 2017 882 4 View

How can I calculate the Performance Coefficient (Cp) from Ansys Fluent?

29 July 2015 1,355 7 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View