Is there any other cell based assay for anticancer study from natural product (plant material)?

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How to extract the metastable conformations from the Free energy landscape graph of protein ligand complex ???

I performed protein ligand complex simulation in gromacs. To obtain PCA and FEL graph, used gmx covar, gmx anaeig and gmx sham module of gromacs. For further analysis, I am in need of lowest...

12 June 2023 1,217 0 View

How to give BPSK modulator as input to a circuit in cadence?

Importing matlab code to cadence.

18 December 2022 3,740 2 View

Suggestions to label a primary antibody for IHC?

I need two proteins co-labeled. Unfortunately, both are are polyclonal antibodies with rabbit as the host. I am trying to look for easy solutions to label one of them with a fluorophore to avoid...

11 January 2022 853 2 View

Metal oxides decorated gold nanorods/ Gold nanorods coated metal oxide through solution process?

Is there any one pot synthesis method to fabricate gold nanorods coated metal oxide through wet chemical route? I have been trying to fabricate AuNR coated gallium oxide but not able to attain...

19 December 2021 4,666 3 View

Can anyone suggest the Second-Life of Used EV Batteries for a small-scale project?

I am trying to do a project with the Second-Life of Used EV Batteries for my university. I have the batteries in hand and even teardown them. Please suggest me am idea like power grid usage or...

31 July 2021 9,725 3 View

Can anyone suggest a good research topic or a potential project regarding Embedded system or control engineering on a Electric vehicle?

I'm looking for some good suggestions to do a decent project in a EV vehicle where I'll be able to do reverse engineering and analyse the components used in it. The project span will be around 2...

12 June 2021 174 7 View

How many references to cite for a review article?

I am currently working on a review article. however, I am not able to get more research papers for it. Still my article is quite having content as the data is very rare. Is there any minimum...

17 May 2020 4,008 20 View

How does one mine data from the Cancer Therapeutics Response Portal?

I am interested in using the Broad's Cancer Therapeutics Response Portal to see the correlation between the expression of a particular gene of interest and resistance to a few specific drugs. I...

23 February 2020 254 3 View

How to change the pre-trained Caffe model to accept one channel input(Gray Scale Image) instead of an RGB image?

I apply holistically nested edge detection method for Edge and Boundary Detection in Images. The model can accept only the RGB image. Can anyone help me to accept one channel input image(Gray...

27 January 2020 5,609 3 View

How crucial or disadvantageous is an emergence of regional security alignment in the Indo-Pacific region ?

The security environment in the region is uncertain and evolving. Though the bilateral and trilateral defense interactions are increasing, might this lead to the emergence of a new regional...

15 December 2019 7,070 3 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View