How can I Model a Direct Drive PMSG Wind Turbine ?

Hello All, I am trying to test the performance of a motor(CMP80M - Sew Eurodrive - PMSM) as a generator in case of a Wind Turbine system. I have already implemented the dq equations of a PMSG for...

29 April 2019 9,445 1 View

Porous media combustion in FLUENT or CFX ?

How to do simulation in PMC to get mass fraction of hydrogen from Biomass combustion using FLUENT or CFX with cad model of combustor?? Is there any specif papers available.. help me

02 April 2019 5,071 2 View

What are the possible phytochemical groups that can get soluble in ethanol and hexane extracts?

During extraction of herbal material in ethanol and hexane what are the group of phytochemicals that we can expect to get soluble in it?

21 March 2018 3,120 7 View

Someone there doing colchicine extraction from Gloriosa superba?

How difficult the extraction process is? Can anyone share me the working protocol to learn the procedure? To establish a start up, what all are the required equipment initially?

01 November 2017 8,380 2 View

A right circular polarized(RCP) light changes to LCP after reflection from a medium of higher ref. index, but does it differ with angle of incidence?

Actually my concern is for the reflection from a Silver/Aluminium mirror, at an angle of incidence of 45 degrees. And the wavelength is in the visible range.

22 February 2015 8,285 7 View

How do I take my methanol soluble substance to MTT assay to test the effect of this substance on my cell cultures?

The unidentified substance of my interest derived from a plant is only methanol soluble. how would I do MTT assay using this substance of my interest?

27 June 2014 6,725 3 View

Can someone suggest a mechanical device with two inputs for pressurized gases and one outlet with constant pressure output?

I need 2 gases with diff. pressures to enter into a chamber, combine and come out with a required pressure (that required pressure will be set by me). The input pressures may not be always...

01 August 2013 3,285 9 View

How do we estimate the bioavailability of phytochemicals soluble in pentane?

At times few phytochemicals are soluble only in pentane, so in this case how do we study the bioavailability of those phytochemicals?

05 June 2013 6,096 4 View

Can anyone suggest an inexpensive way of liberating hydrogen from water?

An instantaneous, continuous and pure production is required.

18 July 2012 3,483 15 View

embedded systems

Hi,I am new to the group.I am currently working as a software professional in IT I want to be a embeddded software developer.Can any one tell me how to step into the embedded field?

25 May 2011 3,895 25 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

Has anyone tried to scan fluorescence IHC slides with microarray scanner?

I have an old slide microarray scanner (VIDAR Revolution 4200) and would like to give it a "second life", as IHC scanner array (fluorescence dyes), FISH over tissue or similar. Has anyone tried...

24 February 2021 9,210 1 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

No title

22 February 2021 9,932 11 View

How to locate and collect the pineal gland in the amphibian brain?

Our team has been trying to locate and collect the pineal gland in the American bullfrog's brain (Lithobates catesbeianus) for some time now. However, we don't have a standardized protocol for...

18 February 2021 3,453 4 View