On searching the methods for Rosemary oil profiling we find only GC and GCMS. Does anyone have Rosemary oil profiling using HPLC?
Dear Anjali,
Please read the following publications:
1-Anal Bioanal Chem. 2011 Jul;400(9):3109-23. doi: 10.1007/s00216-011-4976-5. Epub 2011 Apr 16.
Application of high-performance liquid chromatography diode array detection and mass spectrometry to the analysis of characteristic compounds in various essential oils.
Turek C1, Stintzing FC.
Author information
Abstract
A high-performance liquid chromatography (HPLC) method was established using an analytical reversed-phase column and gradient elution to achieve chromatographic separation of typical compounds in essential oils. For detection, a diode array detector monitoring different wavelengths simultaneously as well as a mass spectrometer (MS) were used. Atmospheric pressure chemical ionization operating in the positive mode turned out to be a suitable tool to detect volatiles of different chemical classes and to identify them in essential oil matrices. Characteristic fingerprints of eucalyptus, lavender, may chang, pine, rosemary, thyme, and turpentine essential oils monitored at a representative wavelength (220 nm) demonstrated the suitability of HPLC in essential oil analysis. Additional monitoring wavelengths (210, 250, and 280 nm) provided useful information about the identity of the specific component and opened the possibility to differentiate presumably coeluting compounds by means of their distinct absorption behavior. Finally, peak assignment in seven essential oils was performed on the basis of characteristic retention times and UV and MS data of a broad set of reference volatiles.
http://www.ncbi.nlm.nih.gov/pubmed/21499677
2-Fast high performance liquid chromatography and ultraviolet–visible
quantification of principal phenolic antioxidants in fresh rosemary
N. Troncoso a, H. Sierra a, L. Carvajal a, P. Delpiano a, G. Gunther ¨ b,∗
a Fundaci´on Chile, Area Agroindustria, Parque Rabat 6165 Vitacura, Santiago, Chile b Universidad de Chile, Facultad de Ciencias Qu´ımicas y Farmac´euticas,
Departamento de Qu´ımica Org´anica y Fisicoqu´ımica, Casilla 233, Santiago–1, Chile
Abstract
An improved HPLC method is reported for the determination of rosemary’s principal phenolic antioxidants, rosmarinic and carnosic acids, providing a fast and simultaneous determination for both of them by using a solid phase column. The analysis was performed with fresh methanolic extractions of Rosmarinus officinalis. To quantify the amount of antioxidants in a fast and reproducible way by means of UV–vis absorption measurements, a spectrophotometric multi-wavelength calibration curve was constructed based on the antioxidant contents obtained with the recently developed HPLC method. This UV–vis methodology can be extended to the determination of other compounds and herbs if the restrictions mentioned in the text are respected.
http://repositorio.uchile.cl/bitstream/handle/2250/120411/Troncoso_N.pdf?sequence=1
3-Prevention of retinal light damage by zinc oxide combined with rosemary extract
Analysis of Actives
Introduction
While not a thorough investigation of bioavailability of the different constituents of rosemary, this analysis provides assessment of the total amount of its primary antioxidant components. The analysis does not distinguish amongst dissolved, solubilized, suspended-microparticulate or carrier-bound forms. The bioresponses reported in the paper establish significance from their total measured amounts. For purposes of comparison across species, data are presented in terms of mg/kg, as are the vitamin / mineral components in the AREDS formulation.
http://www.molvis.org/molvis/v19/appendices/mv-v19-1433-app-1.pdf
Hoping this will be helpful,
Rafik
Dear angali,
Hi,
I suggest to correspond with Dr. Fatemeh Sefidkon through her researchgate profile, and ask her to send you wealthy information about rosemary and other plant oils constituents. She has done many researches on plant oils in Iran. Follow the below link:
https://www.researchgate.net/profile/Fatemeh_Sefidkon
Best regards
Why you want a HPLC analysis for essential oil? In my opinion, capillary based gas chromatography is best technique for separation of constituents in essential oils. Meanwhile, it shall be more appropriate if HPLC analysis is used for detection of carnosic acid, rosemarinic acid or similar compounds in rosemary extract.
Good Luck
Sir do you know any method to purify rosmarinic acid? The spec what we obtain should near 30-60% purity? Anything other than ethyl acetate ?
Sir do you know the method for determination of chemical constituents in lavender oil and tea tree oil except GC-MS?
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