Whats the best and cheaper source of Trichodermin is it harmful?

Are there any simpler extraction techniques and is it harmful?

10 November 2016 7,050 0 View

Whats the simplest method to stain yeast cells?

whats the simplest method to stain yeast cells? is Gram staining effective?

07 August 2016 4,489 3 View

Whats the exact method to do the MIC test of Malasseziafurfur?

whats the exact method to do the MIC test of Malasseziafurfur? Can we do it in SD broth with 1% olive oil? Or is it mandatory to do it on agar? Which s the best? Can someone share the exact...

06 July 2016 4,085 0 View

What is the dosage of E392 Rosemary extract in cheese? What is new regulation of E392 Rosemary in food application?

Please get the PDF sources if any

06 July 2016 7,522 1 View

Whats the microbial limit in fruit juices?

when we prepare different juices and beverages. whats the microbial limit and which regulations to refer?

06 July 2016 9,313 2 View

What is the usual size of an enzyme? How to remove the enzymes from a reaction mixture easily?

06 July 2016 4,372 3 View

What are the plants that best sources of black color ?

are there any plants or seeds common that are best sources of black color? whats its pigment chemistry?

05 June 2016 6,008 7 View

How to differentiate fungal and bacterial spores in food products?

How to differentiate the spores and what are the patterns to eliminate them? If its fungal spore how does it affect product nature? any chemicals to kill?

05 June 2016 8,665 3 View

How will you extract pure Nisin/Natamycin from natural source? Anybody has the exact procedure?

Need Food or pharma grade NIsin/Natamycin for checking from microbial sources that are eco friendly?

05 June 2016 3,859 2 View

Are there any building norms for a microbiology lab?

when you set up a new microbiology lab are there any requirements for the building, location, floor or cieling? Which regulations to follow

05 June 2016 9,883 8 View

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02 March 2021 3,060 3 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Is there a method of determining feed digestibility without the need to re-pellet the feed?

I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....

28 February 2021 7,967 3 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View