Whats the best and cheaper source of Trichodermin is it harmful?

Are there any simpler extraction techniques and is it harmful?

10 November 2016 7,050 0 View

Whats the exact method to do the MIC test of Malasseziafurfur?

whats the exact method to do the MIC test of Malasseziafurfur? Can we do it in SD broth with 1% olive oil? Or is it mandatory to do it on agar? Which s the best? Can someone share the exact...

06 July 2016 4,085 0 View

What is the dosage of E392 Rosemary extract in cheese? What is new regulation of E392 Rosemary in food application?

Please get the PDF sources if any

06 July 2016 7,522 1 View

What is the difference between ATCC and MTCC culture?

what is the difference between ATCC and MTCC culture? Will it affect country wise? Any pdf for support?

06 July 2016 4,259 7 View

Whats the microbial limit in fruit juices?

when we prepare different juices and beverages. whats the microbial limit and which regulations to refer?

06 July 2016 9,313 2 View

What is the usual size of an enzyme? How to remove the enzymes from a reaction mixture easily?

06 July 2016 4,372 3 View

What are the plants that best sources of black color ?

are there any plants or seeds common that are best sources of black color? whats its pigment chemistry?

05 June 2016 6,008 7 View

How to differentiate fungal and bacterial spores in food products?

How to differentiate the spores and what are the patterns to eliminate them? If its fungal spore how does it affect product nature? any chemicals to kill?

05 June 2016 8,665 3 View

How will you extract pure Nisin/Natamycin from natural source? Anybody has the exact procedure?

Need Food or pharma grade NIsin/Natamycin for checking from microbial sources that are eco friendly?

05 June 2016 3,859 2 View

Are there any building norms for a microbiology lab?

when you set up a new microbiology lab are there any requirements for the building, location, floor or cieling? Which regulations to follow

05 June 2016 9,883 8 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View