Whats the best and cheaper source of Trichodermin is it harmful?

Are there any simpler extraction techniques and is it harmful?

10 November 2016 7,050 0 View

Whats the simplest method to stain yeast cells?

whats the simplest method to stain yeast cells? is Gram staining effective?

07 August 2016 4,489 3 View

What is the dosage of E392 Rosemary extract in cheese? What is new regulation of E392 Rosemary in food application?

Please get the PDF sources if any

06 July 2016 7,522 1 View

What is the difference between ATCC and MTCC culture?

what is the difference between ATCC and MTCC culture? Will it affect country wise? Any pdf for support?

06 July 2016 4,259 7 View

Whats the microbial limit in fruit juices?

when we prepare different juices and beverages. whats the microbial limit and which regulations to refer?

06 July 2016 9,313 2 View

What is the usual size of an enzyme? How to remove the enzymes from a reaction mixture easily?

06 July 2016 4,372 3 View

What are the plants that best sources of black color ?

are there any plants or seeds common that are best sources of black color? whats its pigment chemistry?

05 June 2016 6,008 7 View

How to differentiate fungal and bacterial spores in food products?

How to differentiate the spores and what are the patterns to eliminate them? If its fungal spore how does it affect product nature? any chemicals to kill?

05 June 2016 8,665 3 View

How will you extract pure Nisin/Natamycin from natural source? Anybody has the exact procedure?

Need Food or pharma grade NIsin/Natamycin for checking from microbial sources that are eco friendly?

05 June 2016 3,859 2 View

Are there any building norms for a microbiology lab?

when you set up a new microbiology lab are there any requirements for the building, location, floor or cieling? Which regulations to follow

05 June 2016 9,883 8 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

Could you please share your experience with me on the type of 96 wells plate for MIC determination: Treated Vs Untreated and Round-Vs Flat-bottomed?

I am working on the MIC determination of various antibacterial agents (both water-soluble and- insoluble) against S. aureus, MRSA, and S. pyogenes. Some guidelines recommend using round-bottomed...

17 February 2021 7,029 3 View

Can 5-FOA Diffuse Through Agar?

I am preparing to do a gene knockout in the filamentous fungi Coccidioides posadasii targeting pyrG/URA3 so that 5-FOA can be used for selection pressure instead of traditional antibiotic...

16 February 2021 169 4 View