Are there any simpler extraction techniques and is it harmful?
whats the simplest method to stain yeast cells? is Gram staining effective?
07 August 2016 4,618 3 View
whats the exact method to do the MIC test of Malasseziafurfur? Can we do it in SD broth with 1% olive oil? Or is it mandatory to do it on agar? Which s the best? Can someone share the exact...
06 July 2016 4,205 0 View
Please get the PDF sources if any
06 July 2016 7,731 1 View
when we prepare different juices and beverages. whats the microbial limit and which regulations to refer?
06 July 2016 9,529 2 View
what is the difference between ATCC and MTCC culture? Will it affect country wise? Any pdf for support?
06 July 2016 4,498 7 View
How to remove the enzymes from a reaction mixture easily?
06 July 2016 4,500 3 View
are there any plants or seeds common that are best sources of black color? whats its pigment chemistry?
05 June 2016 6,131 7 View
Need Food or pharma grade NIsin/Natamycin for checking from microbial sources that are eco friendly?
05 June 2016 3,997 2 View
How to differentiate the spores and what are the patterns to eliminate them? If its fungal spore how does it affect product nature? any chemicals to kill?
05 June 2016 8,904 3 View
We have a problem of spore contamination and the product cannot be heated for longer temp and longer time
05 June 2016 7,702 25 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?
03 August 2024 7,829 0 View
I am currently working on a project that involves extracting cell membranes, for which we disrupt the cells using sonication. During the initial extraction process, we add protease inhibitors to...
30 July 2024 7,077 1 View
I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...
22 July 2024 5,953 6 View
I’m having difficulty achieving high RNA integrity in my samples. Although the 260/280 and 260/230 ratios are satisfactory after RNA extraction, the RNA samples show signs of degradation when...
22 July 2024 155 4 View
hi every one I am making vector construction (for fusion proteins) and in this moment I wanna to amplification of ADAM17 prodomain with PCR. to yet, I couldn't amplified the ADAM17 prodomain with...
21 July 2024 8,660 1 View
Is there a method that I can measure alcohol dehydrogenase activity using NAD+ in enzyme extraction from plants?
17 July 2024 935 1 View
