Whats the simplest method to stain yeast cells?

whats the simplest method to stain yeast cells? is Gram staining effective?

07 August 2016 4,489 3 View

Whats the exact method to do the MIC test of Malasseziafurfur?

whats the exact method to do the MIC test of Malasseziafurfur? Can we do it in SD broth with 1% olive oil? Or is it mandatory to do it on agar? Which s the best? Can someone share the exact...

06 July 2016 4,085 0 View

What is the dosage of E392 Rosemary extract in cheese? What is new regulation of E392 Rosemary in food application?

Please get the PDF sources if any

06 July 2016 7,522 1 View

What is the difference between ATCC and MTCC culture?

what is the difference between ATCC and MTCC culture? Will it affect country wise? Any pdf for support?

06 July 2016 4,259 7 View

Whats the microbial limit in fruit juices?

when we prepare different juices and beverages. whats the microbial limit and which regulations to refer?

06 July 2016 9,313 2 View

What is the usual size of an enzyme? How to remove the enzymes from a reaction mixture easily?

06 July 2016 4,372 3 View

What are the plants that best sources of black color ?

are there any plants or seeds common that are best sources of black color? whats its pigment chemistry?

05 June 2016 6,008 7 View

How to differentiate fungal and bacterial spores in food products?

How to differentiate the spores and what are the patterns to eliminate them? If its fungal spore how does it affect product nature? any chemicals to kill?

05 June 2016 8,665 3 View

How will you extract pure Nisin/Natamycin from natural source? Anybody has the exact procedure?

Need Food or pharma grade NIsin/Natamycin for checking from microbial sources that are eco friendly?

05 June 2016 3,859 2 View

Are there any building norms for a microbiology lab?

when you set up a new microbiology lab are there any requirements for the building, location, floor or cieling? Which regulations to follow

05 June 2016 9,883 8 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

What is the biochemistry of acidic solvents for phenolics extraction in strawberry?

I am using the Folin-Ciocalteu method for analysis of total phenolic content in ripe strawberries. I've read several protocol that suggest a methanol/H2O/formic acid (80:19:1) extraction solution....

22 February 2021 6,560 3 View

Protocol for membrane extraction E. coli?

Hello! I am interested in determining whether a poly-unsaturated fatty acid I have engineered E. coli to produce is being incorporated into the membrane of the cells or if it is simply a free...

21 February 2021 7,447 2 View

Using 1D CNN for pixel classification in an image?

I have a 3D Image of size 100x100x100. I have features (feature dimension 25) extracted for each pixel by considering a small pacth around it. So the input to 1D CNN is 100x100x100x25 reshaped to...

17 February 2021 941 4 View

Solid Phase Extraction of drug product containing nanoparticles ?

Hi Everyone, I am trying to develop an SPE method for the vaccine which contains Matrix-M (saponin Quillaja Saponaria ) which is in nano-particle form and size is around 40nm. These nanoparticles...

16 February 2021 1,389 2 View

Does anyone know what are the pros and cons for using autoencoders instead of CNNs for features extraction in neural networks?

Are there papers that talk about the possible advantages and disadvantages of using autoencoders instead of CNNs for feature extraction in neural network?

15 February 2021 6,952 3 View

Acid phenol:chloroform (25:24:1) for RNA extraction instead of (125:24:1)?

Hi everyone! I am about to extract RNA for RNA sequencing assays and my Invitrogen kit/protocol...

14 February 2021 1,755 3 View