Whats the best and cheaper source of Trichodermin is it harmful?

Are there any simpler extraction techniques and is it harmful?

10 November 2016 7,050 0 View

Whats the simplest method to stain yeast cells?

whats the simplest method to stain yeast cells? is Gram staining effective?

07 August 2016 4,489 3 View

Whats the exact method to do the MIC test of Malasseziafurfur?

whats the exact method to do the MIC test of Malasseziafurfur? Can we do it in SD broth with 1% olive oil? Or is it mandatory to do it on agar? Which s the best? Can someone share the exact...

06 July 2016 4,085 0 View

What is the dosage of E392 Rosemary extract in cheese? What is new regulation of E392 Rosemary in food application?

Please get the PDF sources if any

06 July 2016 7,522 1 View

What is the difference between ATCC and MTCC culture?

what is the difference between ATCC and MTCC culture? Will it affect country wise? Any pdf for support?

06 July 2016 4,259 7 View

Whats the microbial limit in fruit juices?

when we prepare different juices and beverages. whats the microbial limit and which regulations to refer?

06 July 2016 9,313 2 View

What are the plants that best sources of black color ?

are there any plants or seeds common that are best sources of black color? whats its pigment chemistry?

05 June 2016 6,008 7 View

How to differentiate fungal and bacterial spores in food products?

How to differentiate the spores and what are the patterns to eliminate them? If its fungal spore how does it affect product nature? any chemicals to kill?

05 June 2016 8,665 3 View

How will you extract pure Nisin/Natamycin from natural source? Anybody has the exact procedure?

Need Food or pharma grade NIsin/Natamycin for checking from microbial sources that are eco friendly?

05 June 2016 3,859 2 View

Are there any building norms for a microbiology lab?

when you set up a new microbiology lab are there any requirements for the building, location, floor or cieling? Which regulations to follow

05 June 2016 9,883 8 View

How to calculate the fraction metabolized of a drug by an enzyme using clearance(ml/min) values of the metabolite formed?

I am trying to find the fraction metabolized of a drug by CYP and UGT enzymes. I am unable to get the values of fraction metabolized (Fm) directly from the literature. Can we calculate the Fm of a...

21 February 2021 5,379 2 View

How long does it take to design and validate transition state analogue inhibitors?

Hi, I'm asking from the point of view of designing a research plan: How long (approximately) can it take to determine the transition state structure of an enzyme substrate using kinetic isotope...

14 February 2021 2,610 1 View

Kinetics of Enzyme production in Recombinant E Coli?

Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...

12 February 2021 1,386 3 View

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Hello! I want to determine the activity of my in house Reverse Transcriptase enzyme. For this, I have used Thermo's Enzcheck Reverse Transcriptase assay kit. I used commercial Thermo RT enzyme to...

08 February 2021 7,285 1 View

How define one baker unit(BU) is the amount of hydrogen peroxide catalase ?

07 February 2021 2,006 1 View

Does DNA A-tailing survive after agarose gel electrophoresis and purification?

Hi everyone, I need to clone in pGEM-T vector the result of amplifying a metagenomic library by PCR with a high fidelity polymerase but I only want to clone the fragments of an specific size...

04 February 2021 6,051 8 View

What are some common oxidants used to oxidize iron sulfur clusters in proteins for techniques like EPR?

I have used potassium ferricyanide (which is likely the most common) extensively in the past, but I am curious to know if there are other oxidants that work well. I need to generate the oxidized...

29 January 2021 9,803 4 View

Papain Enzyme Assay...?

Hi all, I would like to know how can I estimate the activity of the enzyme, papain, which is used in reactions as crude latex extract of papaya. Please share some procedures if anybody having...

26 January 2021 569 4 View

Where can I find plants pyr-GC-MS data?

25 January 2021 5,403 2 View

Recombinant restriction enzyme: how strong should the corresponding methyltransferase expression level be?

Hello. We are planning expression of a restriction enzyme in E. coli, and wondering how much of the corresponding methyltransferase is needed to be expressed in order to protect the host genome...

23 January 2021 2,096 4 View