Whether there is any effect of heat on phenol and flavonoids concentration if the samples are kept in hot water bath for evaporation of ethanol?
If you are extracting phenolics and flavonoids, you must make sure that the processing method does not alter them in any form.Your method of evaporating ethanol is not optional as you are using 100 or close to 100 degree centigrad. We normally try not to exceed in the region of 55. You can use rotary evaporators followed by freeze drying..
You can use rotatory evaporater to evaporate the solvent.Tempature around 50-60 degrees is fine for solvent ethanol.
However, for phytochemical analysis you must maintain a temperature less than 50, because many phytochemicals may loss due to high temperature.
If you have rotary evaporator with a good vacuum pump, you can concentrate your ethanol extract at 30-40 degrees. But if water bath is the only option you have, you can concentrate your ethanol extract at 50 degrees Celsius, it will not affect your phenolic and flavonoids concentration, but it will take about two days to dry.
The boiling point of water is 100 degree the ethanol in the test tube will heat to about 90 degree. This will decrease the stability of flavoinds. If you donot have rotary evaporator use heat gun even ut is not the best choice
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