I need assistance with step by step guide of how to construct Chimeric antigen receptor for Immune cells using Snapgene software. Thank you for your help
I am looking for journals that admit the publication of results from applying the IUCN Standard for designing and monitoring Nature-based solutions. Many thanks for considering my request.
23 July 2024 6,444 2 View
Despite not having cells in the media, I am getting purple color. I have tried many troubleshooting methods, varying media types, and even different MTTs from different companies to figure out the...
21 July 2024 9,914 1 View
I'm working with a hydrogel sample and I'd like to perform XRD analysis. Can anyone offer guidance on preparing the hydrogel for the XRD instrument? Specifically, I'm unsure about the best method...
20 July 2024 3,611 4 View
Hello everyone, I am currently working on a project where I aim to analyze color preferences in data visualizations across participants from three different countries. The dependent variable in...
19 July 2024 4,178 2 View
There exists a neural network model designed to predict a specific output, detailed in a published article. The model comprises 14 inputs, each normalized with minimum and maximum parameters...
14 July 2024 2,714 3 View
We are using MEGAshortscript™ T7 Transcription Kit for transcribing gRNAs from linearized plasmid templates. (MSP3485 digested with HindIII) (https://www.addgene.org/140082/) We let the reaction...
01 July 2024 6,342 0 View
Hello ResearchGate Community, How can Abaqus CAE be utilized to assess the variation in wall thickness throughout a plastic bottle following a blow molding simulation?
26 June 2024 9,281 4 View
Why the IF of almost all journals declined so much? For the file download go to https://www.researchgate.net/post/JCR_Impact_Factor_2024-Factors_causes_decline_in_IF
21 June 2024 3,358 0 View
When I examined the GIXRD data obtained recently, I observed that the sample treated with O2 plasma on the Cu surface deposited on Si chips showed increased intensity compared to the sample...
19 June 2024 6,900 5 View
I am working on an acetyltransferase that is highly unstable. Its pI is 6.65, and its molecular weight is around 18 kDa. The protein elutes at 1M imidazole and begins to precipitate immediately...
19 June 2024 2,590 0 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Hello! I planned to do an multiplex RNAscope fluorescent assay for cultured cells in a 96 well plate format according to the protocol provided by ACD:...
22 July 2024 3,334 0 View
This question aims to explore the intersection of artificial intelligence and autonomous vehicle technology. It seeks to identify the key challenges faced in implementing AI for recognition...
20 July 2024 3,469 2 View
hi, I am facing difficulties in the développement of an ELISA sandwich. I have several experiences in developing ELISA but that’s the first time I can’t find any solution. My test is currently as...
16 July 2024 9,044 10 View
I try to understand the ESP mechanism working. What kind of sensors it has, Is it necessary to modify or boost its mechanical ?
15 July 2024 4,772 1 View
Is there any benefit in knocking out the interferon receptor in 293T cells? In the context of creating RNA viruses 📷
13 July 2024 3,382 0 View
https://youtu.be/5MckkqF9XWU?si=MvBsElJZg8_At8qP
09 July 2024 6,038 5 View
Hello, I've been doing free floating IHC using a VAChT antibody with a 1:1k dilution (139 103 - Synaptic Systems). However, there has been a struggle to keep an even stain with my samples of...
06 July 2024 4,521 2 View
I would like to know why do people buy temporary things like cars, houses, land, animals etc. when they cannot have it permanently after they die?
05 July 2024 2,715 4 View
What is safe gasoline and Ethanol safe or Ethanol petrol mixture safe for car fuelin.
03 July 2024 2,939 3 View