I need assistance with step by step guide of how to construct Chimeric antigen receptor for Immune cells using Snapgene software. Thank you for your help
I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...
02 March 2021 4,362 1 View
Have a nice day everyone, I'm stuck in extracting datasets out of MPI-ESM-MR netcdf files because the latitude ranges from -3*10^6 to 3*10^6 and longitude ranges -5,9*10^6 to 5,9*10^6. Plus, the...
28 February 2021 912 3 View
Hi everyone, I am currently running a mixed-mode simulation of a DCB using a user-defined cohesive zone model (UEL), however, the results I am getting don't make sense. I used the same UEL for...
24 February 2021 4,157 1 View
We have recently purchased a triaxial test instrument (MATEST) for testing different types of soil. After installing software, we are facing a issue of saturation where the samples does not...
23 February 2021 7,194 4 View
I would like to use some photo from a government website for my questionnaire. But, different photo is from the different owner. So, should I just get permission from that government body or still...
23 February 2021 1,631 3 View
There are contradictory research papers about the correlation between meteorological parameters and incidence of COVID-19 in some countries worldwide. Is there correlation between the...
15 February 2021 5,806 2 View
Of course, there are verity of material, but i need essential material and buffers for this technique. maybe there are some condition for reagent selection that i dont know them. Thank you!
17 January 2021 2,823 13 View
I am conducting a state-of-the-art analysis of these concepts. If you have contributions, please, send me.
17 January 2021 1,123 3 View
I am measuring the crop water use (ET) by using pots and lettuce crop as plant material, all the data of daily weighted pots are ready but I want to find the surface area of each pot. Can you...
12 January 2021 2,482 4 View
I am measuring the crop water use (ET) by using pots and lettuce crop as plant material, all the data of daily weighted pots are ready but I want to find the surface area of each pot. Can please...
12 January 2021 1,726 3 View
We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...
01 March 2021 3,355 3 View
When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...
28 February 2021 2,133 3 View
I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...
25 February 2021 6,635 5 View
This might sound very basic question, but please share with me an explanation of how the T cell is activated when the antigen is not a peptide? What happens? And what are the molecules involved in...
21 February 2021 564 3 View
I have tried to sort CD8+ CXCR5+ in the ARIA but the purity of my sample is about 50%. After sorting, I stain my samples to verify purity but it is low. So, I thought about "downregulation" or...
15 February 2021 8,034 7 View
Hello, I have recently run a western of Vero cells for ACE2. I detected ACE2 at the correct size, but it presents as a double band. The sample was prepared by lysing cells in 1X Laemmli...
07 February 2021 6,973 3 View
Is it possible to attain IC50 if my target cells have less than 50% antigen expression ? For example: Cancer cell line XXX is showing 35% of expression of cell surface antigen YYYY, if i...
04 February 2021 2,711 3 View
Hello, I am conducting a sandwich-type ELISA. It is to see if small molecules can interfere with a protein-protein interaction. As this interaction would occur in the human body, may I heat...
31 January 2021 4,271 3 View
Currently, I am working with RAW BLUE cell lines to check the SEAP expression on addition of TLR ligands? Method developed for different TLR ligands. How experiments can design for validations?...
24 January 2021 589 2 View
Greetings In a theoretical perspective, I want to use a human-derived peptide as a plausible antigen but the problem associated with tolerance hampers my approach. Then I thought why not denature...
20 January 2021 2,201 1 View