11 November 2014 10 6K Report

Hello,

I am trying to cleavage my protein from GST-tag by using Thrombin with its site ( L-V-P-R-G-S) which is provided by plasmid. However, I am confused because I saw after running my protein sequence in ExPASY tool that my protein has also a cleavage site for thrombin (arg-gly). So is the R-G amino acids seq enough to cleavage by thrombin?

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