How to measure or check autoantibodies in mice?

08 September 2018 9,192 1 View

MyD88 protein is membrane protein or cytoplasm protein ?

Hi everyone, I know that TLR signal can recruit the myd88 to cell membrance from cytoplasm. Here I observed thatTLR4 protein slightly increased while total myd88 protein expression decreased. Do...

02 March 2018 8,589 2 View

What is the signal for specific secretion of IFNg/IL17 by different immune cells ?

Hello everyone, Many immune cells in mice colon can secret IFNg and IL17, for example, NK cell, CD4T and CD8 T, they all can secret the IFNg and IL17. Here I want to ask, are the IFNg or IL17...

01 February 2018 5,271 4 View

Are there any colitis mice model that not mediated by CD4 T cells?

Hello everyone, Here I am confused about the colitis mice model, it seems that colitis mice model are the CD4-mediated (increased Th1 or Th17) colitis model, I wonder whether there are colitis...

09 October 2017 570 8 View

Which gene regulate the MyD88 gene expression?

Hello everyone, Does anyone know which gene regulate the MyD88 gene expression in the intestinal epithelial cells ? or whether PI3K/AKT regulate the MyD88 expression or not? if MyD88...

09 October 2017 2,083 4 View

what will happen if there are impaired Th1 Th17 cell differentiation in the mice intestine?

Hello everyone, Th1 and Th17 cell play a pathogenic role in mice disease model, while play a protective role in healthy mice. Here I want to ask will it cause inflammation if there are reduced...

09 October 2017 7,167 0 View

What is the best timepoint to ckeck the immune cells for DSS-induced acute colitis?

03 April 2017 5,184 3 View

Anyone knows about mast cell activation signal pathway?

10 November 2016 2,209 2 View

Why nearly 50% assignment of mouse fecal bacteria to Family S24-7 ?

08 September 2016 4,810 4 View

Which bacteria or antigen can specifically stimulate the secretion of IgG2a in the mice intestine?

07 August 2016 9,909 0 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

How does the gut microbiome affect the fibrotic process within the liver?

and Can the gut microbe be used for the treatment or diagnosis of liver fibrosis?

02 March 2021 557 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View