How to measure or check autoantibodies in mice?

08 September 2018 9,192 1 View

MyD88 protein is membrane protein or cytoplasm protein ?

Hi everyone, I know that TLR signal can recruit the myd88 to cell membrance from cytoplasm. Here I observed thatTLR4 protein slightly increased while total myd88 protein expression decreased. Do...

02 March 2018 8,589 2 View

What is the signal for specific secretion of IFNg/IL17 by different immune cells ?

Hello everyone, Many immune cells in mice colon can secret IFNg and IL17, for example, NK cell, CD4T and CD8 T, they all can secret the IFNg and IL17. Here I want to ask, are the IFNg or IL17...

01 February 2018 5,271 4 View

Are there any colitis mice model that not mediated by CD4 T cells?

Hello everyone, Here I am confused about the colitis mice model, it seems that colitis mice model are the CD4-mediated (increased Th1 or Th17) colitis model, I wonder whether there are colitis...

09 October 2017 570 8 View

Which gene regulate the MyD88 gene expression?

Hello everyone, Does anyone know which gene regulate the MyD88 gene expression in the intestinal epithelial cells ? or whether PI3K/AKT regulate the MyD88 expression or not? if MyD88...

09 October 2017 2,083 4 View

what will happen if there are impaired Th1 Th17 cell differentiation in the mice intestine?

Hello everyone, Th1 and Th17 cell play a pathogenic role in mice disease model, while play a protective role in healthy mice. Here I want to ask will it cause inflammation if there are reduced...

09 October 2017 7,167 0 View

What is the best timepoint to ckeck the immune cells for DSS-induced acute colitis?

03 April 2017 5,184 3 View

Why nearly 50% assignment of mouse fecal bacteria to Family S24-7 ?

08 September 2016 4,810 4 View

Which bacteria or antigen can specifically stimulate the secretion of IgG2a in the mice intestine?

07 August 2016 9,909 0 View

How can I get a single cell suspension from mice intestinal crypt epithelium?

31 December 2015 5,723 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View