MyD88 protein is membrane protein or cytoplasm protein ?

Hi everyone, I know that TLR signal can recruit the myd88 to cell membrance from cytoplasm. Here I observed thatTLR4 protein slightly increased while total myd88 protein expression decreased. Do...

02 March 2018 8,589 2 View

What is the signal for specific secretion of IFNg/IL17 by different immune cells ?

Hello everyone, Many immune cells in mice colon can secret IFNg and IL17, for example, NK cell, CD4T and CD8 T, they all can secret the IFNg and IL17. Here I want to ask, are the IFNg or IL17...

01 February 2018 5,271 4 View

Are there any colitis mice model that not mediated by CD4 T cells?

Hello everyone, Here I am confused about the colitis mice model, it seems that colitis mice model are the CD4-mediated (increased Th1 or Th17) colitis model, I wonder whether there are colitis...

09 October 2017 570 8 View

Which gene regulate the MyD88 gene expression?

Hello everyone, Does anyone know which gene regulate the MyD88 gene expression in the intestinal epithelial cells ? or whether PI3K/AKT regulate the MyD88 expression or not? if MyD88...

09 October 2017 2,083 4 View

what will happen if there are impaired Th1 Th17 cell differentiation in the mice intestine?

Hello everyone, Th1 and Th17 cell play a pathogenic role in mice disease model, while play a protective role in healthy mice. Here I want to ask will it cause inflammation if there are reduced...

09 October 2017 7,167 0 View

What is the best timepoint to ckeck the immune cells for DSS-induced acute colitis?

03 April 2017 5,184 3 View

Anyone knows about mast cell activation signal pathway?

10 November 2016 2,209 2 View

Why nearly 50% assignment of mouse fecal bacteria to Family S24-7 ?

08 September 2016 4,810 4 View

Which bacteria or antigen can specifically stimulate the secretion of IgG2a in the mice intestine?

07 August 2016 9,909 0 View

How can I get a single cell suspension from mice intestinal crypt epithelium?

31 December 2015 5,723 3 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

Has anyone tried to scan fluorescence IHC slides with microarray scanner?

I have an old slide microarray scanner (VIDAR Revolution 4200) and would like to give it a "second life", as IHC scanner array (fluorescence dyes), FISH over tissue or similar. Has anyone tried...

24 February 2021 9,210 1 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View