Milind Mutnale

11 Questions 18 Answers 0 Followers

Questions related from Milind Mutnale

Why Maximum Parsimony in PAUP 4.0 does not work for me?

I am trying to generate MP tree in PAUP 4.0. When I tried to give following command , It does not go accordingly. The addseq will be always 1 during the run. So why this is happening?? any...

08 August 2016 3,551 0 View

Why I get weird curves in qPCR?

some times my standard DNA also gives me weird curves..... I use taqman probes for standard curve analysis... following are some pics attached showing result of qPCR run. Can anybody help me with...

10 October 2015 7,800 8 View

What is a size of ITS region of Fungus??

08 August 2015 2,699 0 View

Can we use regular PCR master mix for qPCR assay?

Can we use regular PCR master mix for qPCR assay? Or we should buy qPCR master mix. I am going to use taqman probe for my qPCR assay.

04 April 2015 6,366 10 View

How to deal with with PCR inhibitors?

how to deal with PCR inhibitors ? what should we add or do to remove PCR inhibitors...

12 December 2014 7,862 7 View

What is the the minimum percentage of identity required by BLAST to tentatively determine new species of bacteria?

I have a 16s rRNA sequence which is showing 96% identity, so it might be a new species?

10 October 2014 4,042 33 View

How can I find out disease causing genes in fungi?

I'm working on a disease causing fungus which only infects amphibians and I would like know which genes from this fungus are responsible for disease. So to do this, how can I proceed? Any method...

09 September 2014 5,807 3 View

What is the minimum DNA quantity (ng) required to visualize DNA on Agarose gel?

I have very low amount of DNA 10 ng/ul. So if I would like to check on gel, how much ul I have to take?

09 September 2014 2,558 40 View

Does anyone have used Nonidet P-40 for Fungal DNA extraction?

Is it also effective on fungal zoospores? I'm trying to extract DNA from B. dendrobatidis Fungi and its zoospore. Can I use Nonidet P-40 to lyse fungal cell wall and zoospore?

09 September 2014 530 1 View

How many pUC18 plasmid DNA molecules will be there in 10 ng/ul conc.?

I would like know how many pUC 18 molecules will be there in 10 ng/ul concentration.. can anyone tell me how to calculate it.???

09 September 2014 3,746 4 View

Can anybody tell me which is the best DNA extraction method for total DNA extraction from cotton swabs?

I'm trying to extract Bd fungal DNA from a cotton swabs after swabbing on frogs. I'm trying to get total DNA, but I'm not getting it. Right now I'm using lysis buffer containing EDTA, Tris HCl,...

09 September 2014 1,630 7 View