How to measure or check autoantibodies in mice?

08 September 2018 9,192 1 View

MyD88 protein is membrane protein or cytoplasm protein ?

Hi everyone, I know that TLR signal can recruit the myd88 to cell membrance from cytoplasm. Here I observed thatTLR4 protein slightly increased while total myd88 protein expression decreased. Do...

02 March 2018 8,589 2 View

What is the signal for specific secretion of IFNg/IL17 by different immune cells ?

Hello everyone, Many immune cells in mice colon can secret IFNg and IL17, for example, NK cell, CD4T and CD8 T, they all can secret the IFNg and IL17. Here I want to ask, are the IFNg or IL17...

01 February 2018 5,271 4 View

Are there any colitis mice model that not mediated by CD4 T cells?

Hello everyone, Here I am confused about the colitis mice model, it seems that colitis mice model are the CD4-mediated (increased Th1 or Th17) colitis model, I wonder whether there are colitis...

09 October 2017 570 8 View

Which gene regulate the MyD88 gene expression?

Hello everyone, Does anyone know which gene regulate the MyD88 gene expression in the intestinal epithelial cells ? or whether PI3K/AKT regulate the MyD88 expression or not? if MyD88...

09 October 2017 2,083 4 View

what will happen if there are impaired Th1 Th17 cell differentiation in the mice intestine?

Hello everyone, Th1 and Th17 cell play a pathogenic role in mice disease model, while play a protective role in healthy mice. Here I want to ask will it cause inflammation if there are reduced...

09 October 2017 7,167 0 View

What is the best timepoint to ckeck the immune cells for DSS-induced acute colitis?

03 April 2017 5,184 3 View

Anyone knows about mast cell activation signal pathway?

10 November 2016 2,209 2 View

Why nearly 50% assignment of mouse fecal bacteria to Family S24-7 ?

08 September 2016 4,810 4 View

How can I get a single cell suspension from mice intestinal crypt epithelium?

31 December 2015 5,723 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

How to plot the probability paper of Gumbel distribution using R programing?

In R-studio, there are many commands of Gumbel package. Arguments are also different. I`m asking about the alpha parameter of the Copula which must be greater than 1. If this is the one used to...

25 February 2021 5,229 3 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View