Hi Otoniel,

   Thanks for your information.

  Do you mean I need to re-assign the S24-7 alone? Thanks!

Xin

Xin, I am not sure how to address this, however we have found the same high percentage of S24-7 in stool from multiple mouse strains, using the Greengenes reference.

-Benjamin

Hi Benjamin,

  If it is, we will lose lots of information. 

  You can try the BLAST approach as Otoniel said.

Xin

Hi Xin et al,

We also see high abundances of this OTU assignment in mice GIT and feces microbiome libraries. We are not alone - There is a pretty thorough discussion of this issue here https://groups.google.com/forum/#!topic/qiime-forum/Ds75aZoVrFY

There are many prokaryotes for which the phylogeny is not well resolved, especially in groups with no isolates, and this appears to be one of those. Makes it a challenge to "name".