How to revive a Hyphomicrobium strain?

11 February 2021 3,524 2 View

Exosome quantification by Elisa based method ?

Hi, I'm working on exosomes isolated from blood samples and cell culture supernatant. I'm wondering if I could quantify exosomes based on Elisa Method coated with exo Ab (CD63 / CD9/C81...) My...

11 November 2020 664 3 View

About exosomes western blotting ?

20 July 2020 1,688 7 View

Which antibodies anti-MSH6 and anti-PMS2 for Western blot analysis on human cell lines ?

Hi everyone, I am new in the field of Western Blot. I would like to perform Western Blot analysis after protein extraction on human cell lines. My two proteins of interest are MSH6 and PMS2. Which...

13 November 2017 5,079 3 View

Potassium acetate role in the aluminum chloride reaction?

I read an article where the Aluminum Chloride Method is used for the determination of total flavonoid content after the reaction between the sample and AlCl3 took place they add potassium acetate....

20 September 2017 3,009 2 View

How can we characterize healthy elderly?

When studying some metabolic parameters in elderly people, it seems difficult to distinguish which ones are caused by aging per se or by age-related diseases. In particular, circulating IL-6 is...

19 June 2014 5,373 5 View

How to detach adherent insect cells?

I have started some insect cells cultures and decided to go with adherent cultures. The protocol that came with the cells (Sf9) presented both the adherent and suspension cultures methods so I did...

25 December 2013 314 10 View

Does anyone know literature about an immune tolerance process to allogeneic father antigens in multiparous female rodents?

Along with the allogeneic foetus tolerance at the maternal interface is there some remanence of this mechanism that renders the mother less reactive when receiving a graft of father cells?

28 November 2013 9,288 3 View

Issue with estradiol inucble system in Arabidopsis

We are working with XVE system from Chua's lab and have encountered a serious problem of induction with estradiol: inducibility of trangene expression in whole leaves from adult plants is...

28 June 2012 9,898 7 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

How climate change will influences human and animal behavioral change?

The role of climate change influencing the human behavior as well as economics interest

01 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View