Hello everyone,
I would like to culture muscle cells, specially Human Umbilical Aortic Smooth Muscle Cells (HUASMCs) in DMEM. There are various papers out there where this has been done, however, they do not comment on whether the DMEM was supplied with any supplements (e.g. NEAAs, sodium pyruvate, glucose...), only whether additional components were added such as FBS or pen strep.
Does anybody here have experience culturing HUASMCs in DMEM, and if so, did it contain any supplements or was it a basal mixture?
Thanks,
Andrew