What is the best technique to identify native bacteria groups present in soil?

I am lay in this subject. Is DNA sequencing the best way to identify the different types of soil bacteria present in soil samples? Ideally, I'd like to find a technique that allows me to identify...

29 September 2020 4,609 9 View

Do you have any suggestion about constrained estimation of mixed models using lme4 package of R?

I would like to use the lmer function of the lme4 package to estimate the random part of the model given the coefficients of the fixed part (which have been estimated in a previous stage using...

24 February 2020 1,731 5 View

Can I use Nanostring microRNA count date (normalized or raw data) to measure relative gene expression?

Can I use Nanostring microRNA count data (normalized or raw data) to measure relative gene expression by comparing it with an endogenous control (mRNA) expression?

25 November 2019 277 2 View

ERT Optimisation protocol sequence software-code for 3D unconventional electrode arrays?

Anyone know about any freely available software or code that can optimise protocol sequences for 3D unconventional electrodes setups, including borehole electrodes? The closest research I found...

15 November 2019 7,802 3 View

How can I calculate the concentration of 30nM miRNA from a 5nMol stock?

I have a transfection experiment to be done. The stock miRNA is 5nMol. The manufacturer recommends adding 75uL of sterile water, giving me a concentration of 66.67 uM. How can I have a final...

05 May 2019 4,141 7 View

Do we have cell culture contamination?

We are experienced lately some problems with primary cells. We could see (even in 10x magnification) tiny bright particles around the addhered cells. There is no macroscopic sign of...

14 December 2016 7,464 11 View

Could anyone help me with PCR to Mycoplasma detection?

I'm tryint to standardize a PCR to mycoplasma detection of dna from cell culture. I'm testing samples positive for mycoplasma and negative as controls. I found a weak band unspecific slightly...

29 June 2016 7,818 3 View

Low caspase activity in heavy metal lethal concentration 50 (LC50). What does that mean?

02 June 2016 1,480 3 View

Can anyone help me to calculate the NP ratio considering that I have mass per phosphate of 13,300 g/mol and mass per amino of 43 gm/mol?

Can anyone help me to calculate the NP ratio considering that I have mass per phosphate of 13,300 g/mol and mass per amino of 43 gm/mol?

22 May 2016 9,403 1 View

How do I read the results of an ANOVA (2 independent factors) that instead of F reason, I got a Z? What is this Z?

I ran a 2 independent factors ANOVA in SPSS and got a Z score instead of F. Is that related to different sample sizes in different conditions? Do I need to even the sizes of the samples before...

08 March 2016 3,813 7 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

Bought HUVEC from Invitrgen.need to revive it.expected it is cryofreezed in DMSO.Do I need to remove the DMSO for plating the cell for the 1st time?

After I cryofreeze the cell do I need to remove the DMSO for culturing the cell for next passages .

28 February 2021 7,738 3 View