Hi there,

The following should help:

http://cdn.intechopen.com/pdfs-wm/39825.pdf

Are you trying to replace a wt gene with mutated one in bacteria using homologous recobination? 

Hi,

The link below may help you:

http://www.intechopen.com/books/cell-interaction/enhancement-of-homologous-recombination-efficiency-by-homologous-oligonucleotides

Hi....He Dongchang

I would say the homology in nucleotide sequence increases the chances for homologous recombination. Depending upon purpose for which your using homologous recombination you can use cre lox system or CRISPR or AC/DS based systems to achieve it.

For fungi knocking out a gene involved in non-homologous integration nku greatly increases homologous recombination