Sachin A. More

4 Questions 56 Answers 0 Followers

Questions related from Sachin A. More

Why a previously protein expressing same gene construct in pET vector (BL21 host) is not showing protein expression?

I am facing problem in protein expression from the gene cloned in pET28 vector (BL21 host) which was previously expressing happily. Now I am trying to induce protein from same construct in same...

11 June 2015 758 10 View

How do I identify correct recombination events worked out using RDP 4 software?

I am trying to find out recombination among viral sequences using RDP 4 software. With the aid of this software I identified two about 30 recombination events. Out these 30 recombination events...

09 March 2015 8,874 21 View

Can we get more than one dissociation curve during real time PCR for one sample contaminated with genomic DNA from other sources ?

I just want to know whether the real time PCR will show different denaturation curves for single sample contaminated with different genomic DNA from different organismal sources? Suppose I have...

01 April 2014 3,881 4 View

How to overcome nuclease activity in E. coli BL21 DE3 while isolating plasmid?

I am trying to isolate the plasmid from E. coli BL21 DE3 in an expression host. The concentration of plasmid DNA is high if I isolate it with boil lysis method but the yield is poor. Another...

28 September 2012 7,430 5 View