Can I perform FACS doublet discrimination in a heterogenous population of cells?

My samples are a mix of cancer cells and lymphocytes. I usually do doublets discrimination when performing data analysis but I would like to know if a heterogeneous doublet formed by a cancer cell...

03 November 2018 7,091 5 View

Which are the main research gaps in Water Harvesting?

Dear all, this is a major question and I hope to start a fruitful discussion. Which are, in your view, the main research gaps in the field of water harvesting? Would you mind to provide recent...

21 December 2017 8,178 11 View

Which MATLAB equation solver is more suitable?

I have a system of 16 variables of non linear and non-smooth equations (there are some min/max functions).Which solver is more suitable?

21 January 2017 3,859 4 View

What is the best way to interpolate raingauges data over mountain areas?

08 August 2016 8,844 8 View

Does anyone know a way to convert the World Reference Base for Soil Resources to the hydrological soil group?

Does anyone know a way to convert the World Reference Base for Soil Resources classification to the hydrological soil group? In particular I would need a conversion table to SWAT soil classification

21 July 2016 2,742 7 View

Hydrology and Water Management Research in Mozambique

Dear all, I am actually in Mozambique working on a rural development project. My department is actually involved as partner to do assessment and design activities, but we would love to insert some...

01 January 1970 6,700 3 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

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02 March 2021 3,060 3 View

12% ledeburitic cold work tool steel, etched for revealing retained austenite, any help for clarifying?

Hello everyone. Given microstructure image belongs to a 12% ledeburitic cold work tool steel (annealed and tempered). The sample was etched with picral (100 mL ethanol and 4 g picric acid). I...

02 March 2021 4,119 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View