Hello,
On sigma website, there is Trizma/Tris buffer table recipe for mixing tris base and tris-hcl for specific pH range between 7.2-9.0, without need for adjust pH. I am looking for the recipe for lower pH. Is there any available? If not, is there any tool to calculate it?
Thank you
Hi there,
Tris buffering effect is no longer effective when pH is below 7.2 (Tris pKa is around 8.2 and buffering effect is effective between pKa-1 and pKa+1) so there is no point in trying to set up a table : pH value would be too variable.
Hi there,
Tris buffering effect is no longer effective when pH is below 7.2 (Tris pKa is around 8.2 and buffering effect is effective between pKa-1 and pKa+1) so there is no point in trying to set up a table : pH value would be too variable.
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