Is there a specific DNA sequence that could be used to distinguish between HPV 16 and HPV 18 ?

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My miniblot transfer system is overheating and proteins are not transferring ?

I recently bought a western mini blot system from invitrogen, The gel runs fine but when I try to transfer it the sponge pads and filter paper get really hot but the proteins don't actually...

06 July 2018 9,165 0 View

How many cells do I need to harvest to run a western blot?

I need to run a western blot of some mesenchymal stem cells and was wondering how many cells I need to run a to run a western blot Thank you

02 March 2018 1,092 4 View

Is there a human cell line that is is infected with hpv-16 and both the original and the infected cell lines are available. Thank you

I am interested in studying the gene expression changes associated with hpv-16 in an human cell line but would like to have a control cell line as close as possible to the infected cell. I know...

04 May 2017 2,010 4 View

Can I use a PCR product with a 0.5 260/230 ratio for sequencing?

Hello everyone I am running a pcr of some genomic DNA and it seems to amplify the genes I looking for fine however when I check the sample in the nanodrop I get a curve like in the picture 260/280...

01 February 2015 3,375 5 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Yoke Kqueen Cheah

Please kindly refer attachment below:

Article Rapid real time PCR to distinguish between high risk human p...

Article Detection of human papillomavirus 18 in cervical cancer samp...

http://jcm.asm.org/content/46/10/3437.full

Thanks

Benjamin THOHA Thomas

Kindly go through this

Rapid real time PCR to distinguish between high risk human papillomavirus types 16 and 18 by

H A Cubie,1 A L Seagar,1 E McGoogan,2 J Whitehead,1 A Brass,2 M J Arends,2 and M W Whitley3

Aamal Ghazi Mahdi Al-Saadi

http://jcm.asm.org/content/40/3/863.short

Andres Stucky

Awesome thank you so much

Andres