My miniblot transfer system is overheating and proteins are not transferring ?

I recently bought a western mini blot system from invitrogen, The gel runs fine but when I try to transfer it the sponge pads and filter paper get really hot but the proteins don't actually...

06 July 2018 9,165 0 View

Is there a specific DNA sequence that could be used to distinguish between HPV 16 and HPV 18 ?

I have some sequencing data from patients with cervical cancer and we have been able to determine that a good number of them, but we are having trouble telling for sure between hpv 16 and hpv 18....

09 October 2017 4,108 4 View

Is there a human cell line that is is infected with hpv-16 and both the original and the infected cell lines are available. Thank you

I am interested in studying the gene expression changes associated with hpv-16 in an human cell line but would like to have a control cell line as close as possible to the infected cell. I know...

04 May 2017 2,010 4 View

Can I use a PCR product with a 0.5 260/230 ratio for sequencing?

Hello everyone I am running a pcr of some genomic DNA and it seems to amplify the genes I looking for fine however when I check the sample in the nanodrop I get a curve like in the picture 260/280...

01 February 2015 3,375 5 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View